Summary of Study ST000890

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000619. The data can be accessed directly via it's Project DOI: 10.21228/M8NT1F This work is supported by NIH grant, U2C- DK119886.

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Study IDST000890
Study TitleIdentification of RXR Ligands
Study TypeIdenfication of Ligands by HPLC-MS
Study SummaryFree fatty acids in mouse plasma were identified and quantified by LC-MS. Through differential feeding and PHZ (phnylhydrazine) dosing, coupled with mass spectrometry, we identified the long chain fatty acid C24:5 as a natural RXRA ligand, which was dynamically increased in concentration in response to hematopoietic stress. Collectively, these data demonstrate that natural RXRA ligands are present and are dynamically regulated in vivo in mouse hematopoietic cells.
Institute
Washington University in St. Louis
DepartmentDiabetic Cardiovascular Disease Center, School of Medicine
LaboratoryMetabolomics Core
Last NameFujiwara
First NameHideji
Address660 South Euclid Ave, St. Louis MO 63110
Emailhfujiwar@wustl.edu
Phone314-747-0494
Submit Date2017-09-22
Study CommentsUnits of measurement:peak area ratio: analyte peak area/peak area of internal standard
Analysis Type DetailLC-MS
Release Date2017-10-22
Release Version1
Hideji Fujiwara Hideji Fujiwara
https://dx.doi.org/10.21228/M8NT1F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001452
Analysis type MS
Chromatography type Reversed phase
Chromatography system Shimadzu 20AD
Column Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex API 4000 QTrap
Ion Mode POSITIVE
Units peak area ratio

Chromatography:

Chromatography ID:CH001021
Chromatography Summary:For DMAPA derivatized Fatty acid analysis. Shimadzu 20AD two pump system was used. Solvent A: 0.1% formic acid in water. Solvent B: 0.1% in Acetonitrile. The initial LC condition was 30% B. The solvent gradient was programmed from 30% B to 99% B in 4 min and was hold for 1 min at the flow rate of 1 mL/min. Then, it was immediately brought back to 30 % B and was hold for 2 min. An Agilent Eclipse XDB-C18 column (3.0 x100 mm x 3.5 ┬Ám) was used for this analysis.
Instrument Name:Shimadzu 20AD
Column Name:Agilent Eclipse XDB-C18 (100 x 3.0mm,3.5um)
Column Temperature:60 C
Flow Gradient:30% B to 99% B in 4 min
Flow Rate:1 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:7 min
Chromatography Type:Reversed phase
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