Summary of Study ST000903
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000627. The data can be accessed directly via it's Project DOI: 10.21228/M8MT2H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000903 |
| Study Title | Pharmacometabolomic analysis of rat hippocampus and plasma in response to chronic stress and albiflorin treament |
| Study Type | Animal study |
| Study Summary | Male Sprague-Dawley (SD) rats were exposed to one random stressor per day for 35 d. Following four weeks of stress exposure, rats with stress-induced depression-like behaviors were treated daily with either saline, fluoxetine or albiflorin for 7 days via intragastric gavage. Rat heparinized plasma was collected after drug treatment. After behavior tests, rats were sacrificed and the hippocampus was collected. Pharmacometabolomic analysis was conducted for both plasma and hippocampal samples |
| Institute | Beijing Wonner Biotech. Ltd. Co. |
| Last Name | Zhang |
| First Name | Zuoguang |
| Address | No.9, Kexing Road, Fengtai, Beijing, Beijing, 101111, China |
| leekefeng@hotmail.com | |
| Phone | 86-10-65670787 |
| Submit Date | 2017-11-16 |
| Num Groups | 4 |
| Study Comments | Animal study |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-02-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001469 | AN001470 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Waters ACQUITY UPLC | Waters ACQUITY UPLC |
| Column | Luna NH2 aminopropyl HPLC | Luna NH2 aminopropyl HPLC |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 6500 QTrap | ABI Sciex 6500 QTrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | AUC | AUC |
Chromatography:
| Chromatography ID: | CH001033 |
| Instrument Name: | Waters ACQUITY UPLC |
| Column Name: | Luna NH2 aminopropyl HPLC |
| Column Pressure: | 2200 psi |
| Column Temperature: | 25 °C |
| Flow Rate: | 0.35 ml/min |
| Injection Temperature: | 4 °C |
| Sample Injection: | 12 µl |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium hydroxide; 20 mM ammonium acetate, pH 9.0 |
| Solvent B: | 100% acetonitrile |
| Sample Loop Size: | 12 |
| Randomization Order: | Yes |
| Chromatography Type: | HILIC |
