Summary of Study ST001073
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000718. The data can be accessed directly via it's Project DOI: 10.21228/M8VT2P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001073 |
| Study Title | Lipid profiling of Wnt3a-induced optic nerve regeneration |
| Study Type | untargeted lipid profiling |
| Study Summary | We analyzed lipid profiles of mouse retina and optic nerve for 2 time points - 7 and 15 days post-crush, and 3 conditions - intact control, optic nerve crush + vehicle (saline) intravitreal injection, optic nerve crush + 20 ng of Wnt3a injection. |
| Institute | University of Miami |
| Department | Ophthalmology, Bascom Palmer Eye Institute |
| Laboratory | Sanjoy K. Bhattacharya lab |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | Bascom Palmer Eye Institute (McKnight Bldg.), 1638 NW 10th Avenue, Suite 707A, University of Miami, Miami, FL, 33136 |
| sbhattacharya@med.miami.edu | |
| Phone | 305-482-4103 |
| Submit Date | 2018-10-03 |
| Num Groups | 6 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-12-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001755 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Accela 600 |
| Column | Thermo Acclaim C30 (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | relative abundance (main area) |
Chromatography:
| Chromatography ID: | CH001239 |
| Chromatography Summary: | Reversed phase chromatographic separation utilized Accela Autosampler (Thermo), Accela 600 pump (Thermo) and Acclaim C30 column: 3 µm, 2.1x150 mm (Thermo). The column temperature was maintained at 30˚C and tray temperature at 20˚C. Solvent A was composed of 10 mM ammonium acetate (LC-MS grade) in 60:40 methanol:water (LC-MS grade) with 0.2% formic acid (FA; LC-MS grade). Solvent B was composed of 10 mM ammonium acetate with 60:40 methanol:chloroform with 0.2% FA. The flow rate was 260 µL/min and injection volume was 10 µL. The gradient was 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min. |
| Instrument Name: | Thermo Accela 600 |
| Column Name: | Thermo Acclaim C30 (150 x 2.1mm,3um) |
| Column Temperature: | 30˚C |
| Flow Gradient: | The gradient was 35-100% solvent B over 13.0 min, 100% solvent B over 13.0-13.8 min, 100-35% solvent B over 13.8-14.5 min, 35% solvent B over 14.5-18.0 min, 0% solvent B over 18.0-20.0 min. |
| Flow Rate: | 260 µL/min |
| Sample Injection: | 10 µL |
| Solvent A: | 60% methanol/40% water; 0.2% formic acid; 10 mM ammonium acetate |
| Solvent B: | 60% methanol/40% chloroform; 0.2% formic acid; 10 mM ammonium acetate |
| Analytical Time: | 20 min |
| Washing Buffer: | methanol |
| Chromatography Type: | Reversed phase |
