Summary of Study ST001113

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000744. The data can be accessed directly via it's Project DOI: 10.21228/M8HD5K This work is supported by NIH grant, U2C- DK119886.

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Study IDST001113
Study TitleExposure to Oral Contraceptives Alters Human Endometrial Stem Cells Culture Media Metabolomics
Study SummaryAlthough the effects of oral contraceptives (OCs) in the endometrium has been well established, its influence in the production and metabolism of endometrial mesenchymal stem cells (EnMSC) remains unclear. Therefore, we analyzed the effect of OCs in the EnMSC secretome by culture media quantitative metabolomics. The EnMSC were collected from menstrual shedding of five donors (OC group, n=3; control, non-OC group, n=2) and cultured for three passages. Cells characterization was performed by flow cytometry, and culture media was collected at the end of each passage for further quantitative metabolomics. The metabolites with higher discriminant power for sample classification were Alanine, PC aa C30:0, c4-OH-Pro, PC aa C32:2, PC ae C32:2, PC ae C40:2, glycine and PC ae C32:1 for the OC group, whereas PC aa C36:6, PC aa C34:4, SM OH C16:1, SM C26:0, PC aa C38:0, serine and PC aa C36:5 were representative of the non-OC group. This panel of metabolites showed 98% of sensitivity in sample classification according with respective groups. Altered concentrations of metabolites may be an effect of OC hormonal properties on EnMSC metabolism. Thus, this metabolomic approach could assist in the management of future stem cell therapies according to patients’ specific responses to hormone treatments.
Institute
Universidade Federal de Sao Paulo
Last NameCordeiro
First NameFernanda
AddressRua Embau, 231, Vila Clementino
Emailfernandabertuccez85@gmail.com
Phone11996667402
Submit Date2018-01-16
Analysis Type DetailMS(Dir. Inf.)
Release Date2019-01-22
Release Version1
Fernanda Cordeiro Fernanda Cordeiro
https://dx.doi.org/10.21228/M8HD5K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001809
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system None
Column none
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex 4000 QTrap
Ion Mode UNSPECIFIED
Units umol

Chromatography:

Chromatography ID:CH001274
Instrument Name:None
Column Name:none
Chromatography Type:None (Direct infusion)
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