Summary of Study ST001134
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000759. The data can be accessed directly via it's Project DOI: 10.21228/M8K67W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001134 |
| Study Title | GC-TOF-MS, Brassica nigra / MeJA + herbivory |
| Study Summary | GC-TOF-MS, Brassica nigra / MeJA + herbivory |
| Institute | Umeå Plant Science Centre |
| Department | Plant Physiology |
| Last Name | Papazian |
| First Name | Stefano |
| Address | Umeå University |
| stefano.papazian@umu.se | |
| Phone | 0046723160854 |
| Submit Date | 2019-02-06 |
| Num Groups | 4 |
| Total Subjects | 55 |
| Study Comments | Measurements are chromatographic peak area normalized by internal standard, arbritrary units. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2019-08-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001859 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890 |
| Column | DB5-MS |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Agilent 6890 |
| Ion Mode | POSITIVE |
| Units | Normalized peak area |
Chromatography:
| Chromatography ID: | CH001346 |
| Chromatography Summary: | Samples were analysed by GC-TOF-MS (on an Agilent 6890 gas chromatograph equipped with a 10 m x 0.18 mm silica capillary column with a 0.18 µm DB 5-MS UI stationary phase, J&W Scientific; connected to a LECO Pegasus III TOF-MS) operated using the LECO ChromaTOF® software package (Leco Corp., St Joseph, MI, USA). Retention indices (RIs) were calculated relative to those of a C8-C40 alkane series. Deconvolution, feature extraction and peak integration were performed using a Matlab® script developed in-house. Identification was achieved by comparing compounds' RIs and mass spectra to the SMC in-house mass spectra library and to the public Golm Metabolome Database developed by the Max Planck Institute (GMD-MPI; http://gmd.mpimp-golm.mpg.de). Samples were normalized with respect to the peak areas of the internal standards (methyl stearate and SA-D4). |
| Instrument Name: | Agilent 6890 |
| Column Name: | DB5-MS |
| Flow Rate: | 1mL/min |
| Injection Temperature: | 270°C |
| Internal Standard: | salicylic acid |
| Internal Standard Mt: | stearic acid |
| Capillary Voltage: | 70-eV |
| Oven Temperature: | 70°C to 320°C (40 °C /min) |
| Running Voltage: | detector voltage 1,700V. |
| Transferline Temperature: | 250°C |
| Chromatography Type: | GC |
