Summary of Study ST001167

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000779. The data can be accessed directly via it's Project DOI: 10.21228/M8097N This work is supported by NIH grant, U2C- DK119886.

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Study IDST001167
Study TitleComprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry
Study SummaryWe developed a stable-isotope tracing capillary electrophoresis (CE)-MS metabolomics approach to cover polar metabolites as well as isotopologues in a non-targeted way. An in-house developed software enables high throughput processing of complex multi-dimensional data. The practicability is demonstrated analysing 13C-U-glucose exposed prostate cancer and non-cancer cells.
Institute
Dalian Institute Of Chemical Physics, Chinese Academy Of Sciences
Last NameWang
First NameZhichao
Address457, Zhongshan Road
Emailwangzc05@dicp.ac.cn
Phone+86-15998625250
Submit Date2019-01-06
Study CommentsComprehensive Profiling by Non-targeted Stable Isotope Tracing Capillary Electrophoresis-Mass Spectrometry - A Novel Tool Complementing Metabolomics Analyses of Polar Metabolites
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2020-01-06
Release Version1
Zhichao Wang Zhichao Wang
https://dx.doi.org/10.21228/M8097N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001929 AN001930
Analysis type MS MS
Chromatography type CE CE
Chromatography system Agilent 1290 Infinity II Agilent 1290 Infinity II
Column a fused silica capillary (50 μm i.d. × 80 cm) a fused silica capillary (50 μm i.d. × 80 cm)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name Agilent 6224 TOF Agilent 6224 TOF
Ion Mode POSITIVE NEGATIVE
Units Relative intensity after normalization Relative intensity after normalization

Chromatography:

Chromatography ID:CH001400
Instrument Name:Agilent 1290 Infinity II
Column Name:a fused silica capillary (50 μm i.d. × 80 cm)
Column Temperature:20
Flow Rate:a positive voltage of 27 kV
Injection Temperature:20
Solvent A:1 M formic acid
Chromatography Type:CE
  
Chromatography ID:CH001401
Instrument Name:Agilent 1290 Infinity II
Column Name:a fused silica capillary (50 μm i.d. × 80 cm)
Column Temperature:20
Flow Rate:internal pressure of 15 mbar and positive voltage of 30 kV
Injection Temperature:20
Solvent A:solution of 25 mM ammonium acetate and 75 mM diammonium hydrogen phosphate (pH 8.5)
Chromatography Type:CE
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