Summary of Study ST001170
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000782. The data can be accessed directly via it's Project DOI: 10.21228/M8M10T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001170 |
| Study Title | Timecourse on MCF-7 cells treated with different concentration of doxorubicin |
| Study Summary | MCF-7 cells treated with 10μM doxorubicin for 4h followed by subsequent withdrawl of the drug and cultured up to 48h.Doxurubicin-treated cells and control cells were collected at 0,12,24,36,and 48h. Meanwhile,MCF-7 cells continuously exposed to a low dosage of doxorubicin at 0.1μM for 96h. Doxorubicin-treated cells and control cells were collected at 0,24,48,72 and 96h. |
| Institute | China Pharmaceutical University |
| Last Name | Shao |
| First Name | Chang |
| Address | Tongjiaxiang #24, Nanjing, Jiangsu, 210000, China |
| cici_shao@126.com | |
| Phone | 13951628679 |
| Submit Date | 2019-04-11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | qgd |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-12-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001935 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Shimadzu LC-30A |
| Column | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | ABI Sciex 5600 TripleTOF |
| Ion Mode | NEGATIVE |
| Units | normalized peak area |
Chromatography:
| Chromatography ID: | CH001404 |
| Chromatography Summary: | The mobile phase consisted of solvent A (5 mM ammonium acetate, pH=9, and 5% acetonitrile) and solvent B (acetonitrile). The gradient was set as follows: 0–3 min, 85% B; 3–6 min, 85–30% B; 6–15 min, 30–2% B; 15–18 min, 2% B; 18–19 min, 2–85% B; 19–26 min, 85% B. The flow rate was set at 0.4 mL/min. |
| Instrument Name: | Shimadzu LC-30A |
| Column Name: | Waters XBridge Amide (100 x 4.6mm,3.5um) |
| Flow Gradient: | 0-3 min, 85% B; 3-6 min, 85-30% B; 6-15 min, 30-2% B; 15-18 min, 2% B; 18-19 min, 2-85% B; 19-26 min, 85% B. |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 5% acetonitrile/95% water; 5 mM ammonium acetate, pH 9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
