Summary of Study ST001185

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000796. The data can be accessed directly via it's Project DOI: 10.21228/M8SM3C This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001185
Study TitleGenetic and metabolic characterization of bioengineered human fatty liver tissue with modified SIRT1 expression
Study SummaryLipidomics and metabolomics was performed three types of tissue samples to compare human normal liver tissue against human NASH liver and the bioengineered human iPS-derived fatty liver tissue-iKD-SIRT1. The purpose of this study was to show that the global lipidomics profile of iPS-derived fatty liver tissue-iKD-SIRT1 was similar to that of patients with NASH
Institute
University of Pittsburgh
DepartmentDepartment of Pathology
Last NameSoto-Gutierrez
First NameAlejandro
Address200 Lothrop Street, 423 Biomedical Science Tower, Pittsburgh, PA 15261, USA
Emailals208@pitt.edu
Phone+14126480064
Submit Date2019-05-20
Num Groups3
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2020-01-06
Release Version1
Alejandro Soto-Gutierrez Alejandro Soto-Gutierrez
https://dx.doi.org/10.21228/M8SM3C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001967 AN001968 AN001969
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Vanquish
Column Thermo Fisher Accucore C18 (100 x 2.1mm,5um) Thermo Fisher Accucore C18 (100 x 2.1mm,5um) Phenomenex Kinetex C18+ (100 x 2.1mm,1.7um)
MS Type ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE NEGATIVE UNSPECIFIED
Units Peak area normalized to internal standard Peak area normalized to internal standard Peak Area

Chromatography:

Chromatography ID:CH001423
Chromatography Summary:LIPIDOMICS: Samples were separated on a Thermo Fisher Accucore C18 column (2.1 X 100 mm, 5 μ pore size) using solvent A (H2O:ACN (1:1) with 10 mM ammonium acetate + 0.1% formic acid) and solvent B (IPA:ACN (9:1) with 10 mM ammonium acetate + 0.1% formic acid) at a flow rate of 0.2 mL/min. The gradient started at 0%B and increased to 50%B from 2-10 min following a second increase to 95%B from 10-47 min. The gradient was held for 4 min at 95%B before increasing to 100%B at 51 min for a 6 min wash. At 57 min the system was returned to initial conditions to equilibrate before the next injection. Total run time was 60 min.
Instrument Name:Thermo Vanquish
Column Name:Thermo Fisher Accucore C18 (100 x 2.1mm,5um)
Flow Gradient:The gradient started at 0%B and increased to 50%B from 2-10 min following a second increase to 95%B from 10-47 min. The gradient was held for 4 min at 95%B before increasing to 100%B at 51 min for a 6 min wash. At 57 min the system was returned to initial conditions to equilibrate before the next injection. Total run time was 60 min.
Flow Rate:0.2 mL/min
Solvent A:50% water/50% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH001424
Chromatography Summary:METABOLOMICS: Briefly, samples were separated over a reversed phase Phenomenex Kinetex C18+ column (2.1 × 100 mm, 1.7 μm particle size) maintained at 40°C. For the 20 minute LC gradient, the mobile phase consisted of the following: solvent A (1.5mM ammonium fluoride) and solvent B (100% acetonitrile). The gradient was the following: 0-12.0 min 5% B, to 100% B, 12.0-15.0 min hold at 100% B, 15.0-15.1 100% to 5% B, 15.1-20.0 min 5%B.
Instrument Name:Thermo Vanquish
Column Name:Phenomenex Kinetex C18+ (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase
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