Summary of Study ST001185
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000796. The data can be accessed directly via it's Project DOI: 10.21228/M8SM3C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001185 |
Study Title | Genetic and metabolic characterization of bioengineered human fatty liver tissue with modified SIRT1 expression |
Study Summary | Lipidomics and metabolomics was performed three types of tissue samples to compare human normal liver tissue against human NASH liver and the bioengineered human iPS-derived fatty liver tissue-iKD-SIRT1. The purpose of this study was to show that the global lipidomics profile of iPS-derived fatty liver tissue-iKD-SIRT1 was similar to that of patients with NASH |
Institute | University of Pittsburgh |
Department | Department of Pathology |
Last Name | Soto-Gutierrez |
First Name | Alejandro |
Address | 200 Lothrop Street, 423 Biomedical Science Tower, Pittsburgh, PA 15261, USA |
als208@pitt.edu | |
Phone | +14126480064 |
Submit Date | 2019-05-20 |
Num Groups | 3 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2020-01-06 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN001967 | AN001968 | AN001969 |
---|---|---|---|
Analysis type | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
Column | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) | Phenomenex Kinetex C18+ (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | UNSPECIFIED |
Units | Peak area normalized to internal standard | Peak area normalized to internal standard | Peak Area |
Chromatography:
Chromatography ID: | CH001423 |
Chromatography Summary: | LIPIDOMICS: Samples were separated on a Thermo Fisher Accucore C18 column (2.1 X 100 mm, 5 μ pore size) using solvent A (H2O:ACN (1:1) with 10 mM ammonium acetate + 0.1% formic acid) and solvent B (IPA:ACN (9:1) with 10 mM ammonium acetate + 0.1% formic acid) at a flow rate of 0.2 mL/min. The gradient started at 0%B and increased to 50%B from 2-10 min following a second increase to 95%B from 10-47 min. The gradient was held for 4 min at 95%B before increasing to 100%B at 51 min for a 6 min wash. At 57 min the system was returned to initial conditions to equilibrate before the next injection. Total run time was 60 min. |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Fisher Accucore C18 (100 x 2.1mm,5um) |
Flow Gradient: | The gradient started at 0%B and increased to 50%B from 2-10 min following a second increase to 95%B from 10-47 min. The gradient was held for 4 min at 95%B before increasing to 100%B at 51 min for a 6 min wash. At 57 min the system was returned to initial conditions to equilibrate before the next injection. Total run time was 60 min. |
Flow Rate: | 0.2 mL/min |
Solvent A: | 50% water/50% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH001424 |
Chromatography Summary: | METABOLOMICS: Briefly, samples were separated over a reversed phase Phenomenex Kinetex C18+ column (2.1 × 100 mm, 1.7 μm particle size) maintained at 40°C. For the 20 minute LC gradient, the mobile phase consisted of the following: solvent A (1.5mM ammonium fluoride) and solvent B (100% acetonitrile). The gradient was the following: 0-12.0 min 5% B, to 100% B, 12.0-15.0 min hold at 100% B, 15.0-15.1 100% to 5% B, 15.1-20.0 min 5%B. |
Instrument Name: | Thermo Vanquish |
Column Name: | Phenomenex Kinetex C18+ (100 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |