Summary of Study ST001210
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000813. The data can be accessed directly via it's Project DOI: 10.21228/M8KX27 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001210 |
| Study Title | Comprehensive UHPLC-MS/MS lipidomics profiling to study effects of betulin on keratinocytes |
| Study Summary | Lipidomics analysis of betulin in human primary keratinocytes to monitor alterations in the lipid profiles induced by treatment with betulin |
| Institute | Eberhard Karls University of Tübingen |
| Department | Institute of Pharmaceutical Sciences |
| Laboratory | Pharmaceutical (Bio-)Analysis |
| Last Name | Calderon |
| First Name | Carlos |
| Address | Auf der Morgenstelle 8 (Haus B), D-72076, Tuebingen, baden württemberg, 72076, Germany |
| carlos.calderon@uni-tuebingen.de | |
| Phone | +49 (0)7071 29 74009 |
| Submit Date | 2019-07-05 |
| Num Groups | 2 |
| Total Subjects | 20 |
| Study Comments | Eberhard-Karls-University Tuebingen |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002014 | AN002015 |
|---|---|---|
| Chromatography ID | CH001457 | CH001457 |
| MS ID | MS001867 | MS001868 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 | Agilent 1290 |
| Column | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um,130Å) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um,130Å) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600 TripleTOF | ABI Sciex 5600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized intensities | Normalized intensities |
Chromatography:
| Chromatography ID: | CH001457 |
| Chromatography Summary: | Analyses were performed by Agilent 1290 Series UHPLC instrument coupled to Sciex TripleTOF 5600+ MS with duospray source and Pal HTC-XS autosampler from CTC. Positive and negative ESI ionization were used in separate LC-MS runs. Conditions: Acquity UPLC CSH C18 (130Å, 1.7 µm, 2.1 mm X 100 mm) column was utilized with Acquity UPLC CSH C18 VanGuard pre-column (130Å, 1.7 µm, 2.1 mm X 5 mm). The mobile phase was composed of 10 mM ammonium formate and 0.1 % formic acid dissolved in 60:40 ACN:H2O (v/v) (A) and 90:10 (v/v) IPA:ACN (B). |
| Methods Filename: | ccalcas_LC-MS.pdf |
| Instrument Name: | Agilent 1290 |
| Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um,130Å) |
| Column Temperature: | 65 |
| Flow Rate: | 600 uL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
