Summary of Study ST001323

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000900. The data can be accessed directly via it's Project DOI: 10.21228/M8BX09 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001323
Study TitleEffect of high-fat diet on serum lipidome in mice
Study SummaryWe analyzed mouse serum samples from a mouse dietary intervention experiment. Briefly, C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed High-fat diet (HFD), 1% deoxycholic acid (DCA) in drinking water, both, or left as control for 9 months. For quality control, 12 TQC samples and 2 blanks were also included in the analysis (total 58 samples and 6 groups). The two treatments were selected to demonstrate the ability of lipidomics to detect gross changes induced by HFD in the serum lipidome, as well as specific/minor changes induced by the secondary bile acid (DCA) through regulation of liver lipid metabolism.
Institute
QIMR Berghofer Medical Research Institute
Last NameMohamed
First NameAhmed
Address300 Herston Road
Emailahmed.mohamed@qimrberghofer.edu.au
Phone+61738453992
Submit Date2020-03-02
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2020-03-20
Release Version1
Ahmed Mohamed Ahmed Mohamed
https://dx.doi.org/10.21228/M8BX09
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002199 AN002200 AN002201
Chromatography ID CH001613 CH001614 CH001615
MS ID MS002045 MS002046 MS002047
Analysis type MS MS MS
Chromatography type HILIC HILIC HILIC
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity Agilent 1290 Infinity
Column Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um) Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um) Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um)
MS Type ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Agilent 6490 QQQ Agilent 6490 QQQ
Ion Mode UNSPECIFIED POSITIVE UNSPECIFIED
Units intensity intensity intensity

Chromatography:

Chromatography ID:CH001613
Chromatography Summary:buffer A contained 50% acetonitrile and buffer B contained 95% acetonitrile in water. Buffers were supplemented with 25 mM ammonium formate (pH 4.6) and 0.1% formic acid.
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um)
Solvent A:50% acetonitrile/50% water; 10 mM ammonium acetate, pH 7.6
Solvent B:95% acetonitrile/5% water; 10 mM ammonium acetate, pH 7.6
Chromatography Type:HILIC
  
Chromatography ID:CH001614
Chromatography Summary:buffer A contained 50% acetonitrile and buffer B contained 95% acetonitrile in water. Buffers were supplemented with 25 mM ammonium formate (pH 4.6) and 0.1% formic acid.
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um)
Solvent A:50% acetonitrile/50% water; 10 mM ammonium acetate, pH 7.6
Solvent B:95% acetonitrile/5% water; 10 mM ammonium acetate, pH 7.6
Chromatography Type:HILIC
  
Chromatography ID:CH001615
Chromatography Summary:buffer A contained 50% acetonitrile and buffer B contained 95% acetonitrile in water. Buffers were supplemented with 10 mM ammonium acetate (pH 7.6).
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent HILIC Plus RRHD (100 x 2.1mm, 1.8um)
Solvent A:50% acetonitrile/50% water; 10 mM ammonium acetate, pH 7.6
Solvent B:95% acetonitrile/5% water; 10 mM ammonium acetate, pH 7.6
Chromatography Type:HILIC
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