Summary of Study ST001336
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000900. The data can be accessed directly via it's Project DOI: 10.21228/M8BX09 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001336 |
| Study Title | Effect of high-fat diet and bile acid treatment on serum and tissue lipidomes in mice |
| Study Summary | We analyzed mouse serum and esophageal tissue samples from a mouse dietary intervention experiment. Briefly, C57BL/6 mice (n=44) were divided into 4 groups (n=11 per group) and fed High-fat diet (HFD), 0.2% deoxycholic acid (DCA) in drinking water, both, or left as control for 9 months. For quality control,TQC samples and blanks were also included in the analysis. The two treatments were selected to demonstrate the ability of lipidomics to detect gross changes induced by HFD in the serum and tissue lipidomes, as well as specific/minor changes induced by the secondary bile acid (DCA) through regulation of liver lipid metabolism. The serum and tissue samples were analyzed using targeted and untargeted lipidomics methods. The targeted serum lipidomics data has previously been uploaded as part of study ST001323. |
| Institute | QIMR Berghofer Medical Research Institute |
| Last Name | Molendijk |
| First Name | Jeffrey |
| Address | 300 Herston Road, Herston, QLD, 4006, Australia |
| jeffrey.molendijk@qimrberghofer.edu.au | |
| Phone | +61738453992 |
| Submit Date | 2020-03-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-04-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002226 | AN002227 | AN002228 | AN002229 | AN002230 |
|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Agilent HILIC Plus RRHD (100 x 2.1mm,1.8um) | Agilent HILIC Plus RRHD (100 x 2.1mm,1.8um) | Agilent HILIC Plus RRHD (100 x 2.1mm,1.8um) | Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um) | Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | QTOF | QTOF |
| MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | UNSPECIFIED | POSITIVE | UNSPECIFIED | POSITIVE | POSITIVE |
| Units | Count | Count | Count | Count | Count |
Chromatography:
| Chromatography ID: | CH001634 |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent HILIC Plus RRHD (100 x 2.1mm,1.8um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001635 |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent HILIC Plus RRHD (100 x 2.1mm,1.8um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001636 |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
