Summary of Study ST001401
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000961. The data can be accessed directly via it's Project DOI: 10.21228/M8GD71 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001401 |
| Study Title | Steady-state metabolomics time course of Saccharomyces cerevisiae mitochondrial fatty acid synthesis (mtFAS) mutants |
| Study Type | Steady-state targeted and untargeted metabolomics time course |
| Study Summary | The goal of this work was to analyze metabolic changes in yeast at various time points with either the oar1 KO or the mct1 knock-out conditions when compared to a time-matched wild-type samples using gas chromatography-mass spectrometry (GC-MS). |
| Institute | University of Utah |
| Department | Biochemistry |
| Laboratory | Rutter |
| Last Name | Berg |
| First Name | Jordan |
| Address | 15 N Medical Drive East RM 5520, Salt Lake City, UT 84112-5650 USA |
| jordan.berg@biochem.utah.edu | |
| Phone | (801) 581 3340 |
| Submit Date | 2020-06-10 |
| Num Groups | 3 |
| Total Subjects | 95 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS |
| Release Date | 2020-06-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002343 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 5977b |
| Column | Phenomenex Zebron AB-5HT (5m) |
| MS Type | EI |
| MS instrument type | HES-MSD |
| MS instrument name | Agilent 5977 |
| Ion Mode | POSITIVE |
| Units | AUC (unitless) |
Chromatography:
| Chromatography ID: | CH001716 |
| Chromatography Summary: | All GC-MS analysis was performed with an Agilent 5977b GC-MS MSD-HES and an Agilent 7693A automatic liquid sampler. Dried samples were suspended in 40 µL of a 40 mg/mL O-methoxylamine hydrochloride (MOX) (MP Bio #155405) in dry pyridine (EMD Millipore #PX2012-7) and incubated for one hour at 37 °C in a sand bath. 25 µL of this solution was added to auto sampler vials. 60 µL of N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA with 1%TMCS, Thermo #TS48913) was added automatically via the auto sampler and incubated for 30 minutes at 37 °C. After incubation, samples were vortexed and 1 µL of the prepared sample was injected into the gas chromatograph inlet in the split mode with the inlet temperature held at 250°C. A 10:1 split ratio was used for analysis of the majority of metabolites. The gas chromatograph had an initial temperature of 60°C for one minute followed by a 10°C/min ramp to 325°C and a hold time of 5 minutes. A 30-meter Phenomenex Zebron AB-5HT with 5m inert Guardian capillary column was employed for chromatographic separation. Helium was used as the carrier gas at a rate of 1 mL/min. |
| Instrument Name: | Agilent 5977b |
| Column Name: | Phenomenex Zebron AB-5HT (5m) |
| Chromatography Type: | GC |
