Summary of Study ST001527
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001028. The data can be accessed directly via it's Project DOI: 10.21228/M8T99V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001527 |
| Study Title | Lung cancer metabolomics analysis |
| Study Summary | This study explored models predictive of staging and chemotherapy response based on metabolomic analysis of fresh, patient-derived non-small cell lung cancer (NSCLC) core biopsies. Prospectively collected tissue samples before initial treatment were evaluated with high-resolution 2DLC-MS/MS and 13C-glucose enrichment, and the data were comprehensively analyzed with machine learning techniques. Patients were categorized as Disease-Control (DC) [encompassing complete-response (CR), partial-response (PR), and stable-disease (SD)] and Progressive-Disease (PD). Four major types of learning methods (partial least squares discriminant analysis (PLS-DA), support vector machines (SVM), artificial neural networks, and random forests) were applied to differentiate between positive (DC and CR/PR) and poor (PD and SD/PD) responses, and between stage I/II/III and stage IV disease. Models were trained with forward feature selection based on variable importance and tested on validation subsets. |
| Institute | University of Louisville |
| Department | Bioengineering |
| Last Name | Frieboes |
| First Name | Hermann |
| Address | Lutz Hall 419 |
| hbfrie01@louisville.edu | |
| Phone | 502-852-3302 |
| Submit Date | 2020-09-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-08-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002548 | AN002549 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Unspecified | Unspecified |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Thermo Accucore HILIC (100 x 2.1mm,2.6um) | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | Single quadrupole | Single quadrupole |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak intensity | peak intensity |
Chromatography:
| Chromatography ID: | CH001866 |
| Chromatography Summary: | Used a reversed phase chromatography (RPC) column and a hydrophilic interaction chromatography (HILIC) column configured to form a parallel 2DLC-MS system |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Thermo Accucore HILIC (100 x 2.1mm,2.6um) |
| Chromatography Type: | Unspecified |
