Summary of Study ST001649

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001055. The data can be accessed directly via it's Project DOI: 10.21228/M8B70D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001649
Study TitleUrinary microbiota and metabolome in pediatric vesicoureteral reflux and scarring
Study TypeObservational/cross-sectional
Study SummaryWe enrolled girls and boys aged zero to nine years presenting to a pediatric urologist for recurrent urinary tract infection (UTI) or renal scarring (decreased uptake on a nuclear renal scan) or grade 3-5 vesico ureteral reflux (VUR). Exclusion criteria included other urogenital abnormalities, medical renal disease, immunodeficiency, syndromes associated with VUR, acute UTI, persistent UTI (ongoing positive urine culture 1-3 weeks after completing a treatment course), or global renal atrophy on imaging. At one patient visit, a urine specimen was collected for 16S and metabolomic analysis.
Institute
University of Missouri-Columbia
DepartmentMU Metabolomics Center
Last NameSarma
First NameSaurav
Address1201 Rollins street, Bond Life Science Center
Emailsarmas@missouri.edu
Phone5738825596
Submit Date2021-01-01
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2021-01-25
Release Version1
Saurav Sarma Saurav Sarma
https://dx.doi.org/10.21228/M8B70D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002695
Analysis type MS
Chromatography type GC
Chromatography system Agilent 6890N
Column DB-5MS (J&W Scientific) 60m
MS Type EI
MS instrument type Single quadrupole
MS instrument name Agilent 5973
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH001988
Chromatography Summary:Gas chromatography integrated to mass spectrometer
Chromatography Comments:1:1 split injection with 1uL injection volume
Instrument Name:Agilent 6890N
Column Name:DB-5MS (J&W Scientific) 60m
Column Pressure:variable
Column Temperature:80 0C for 2 minutes, then ramped at 5 0C /min to 315 ºC and held at 315 ºC for 12 minutes
Flow Rate:1mL/Min of Helium gas
Injection Temperature:280C
Internal Standard:Ribitol
Retention Time:9 to 61 min
Analytical Time:61 min
Oven Temperature:80C to 315C
Chromatography Type:GC
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