Summary of Study ST001680
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001079. The data can be accessed directly via it's Project DOI: 10.21228/M8797T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001680 |
| Study Title | Metabolome of NAFLD in high fat diet mouse model |
| Study Summary | We investigated the effects of a RAR beta 2 agonist, AC261066, on the hepatic metabolites changed in high fat fed NAFLD mouse model. We suggest that AC261066 has potential therapeutic relevance for the prevention/treatment of NAFLD and NASH. |
| Institute | Weill Cornell Medicine |
| Last Name | Gudas |
| First Name | Lorraine |
| Address | 1300 York Ave |
| chl2029@med.cornell.edu | |
| Phone | 646-3094389 |
| Submit Date | 2021-01-27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-01-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002738 | AN002739 | AN002740 | AN002741 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Normal phase | Normal phase | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II | Agilent 1290 Infinity II |
| Column | Cogent Diamond Hydride (150 × 2.1mm,3.5um) | Cogent Diamond Hydride (150 × 2.1mm,3.5um) | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | QTOF | QTOF | QTOF | QTOF |
| MS instrument name | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF | Agilent 6550 QTOF |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | abundance | abundance | abundance | abundance |
Chromatography:
| Chromatography ID: | CH002024 |
| Chromatography Summary: | The mobile phase for ANP separation consisted of 6 mM EDTA and 0.025% acetic acid in isopropanol:H2O (50:50) (solvent A) and 6 mM EDTA and 5mM ammonium acetate in CH3CN: H2O (90:10) (solvent B). Gradient steps were applied as follows: 0–1 min, 99% B; 1–15 min, to 20% B; 15.1–29 min, 0% B; 29.01-35 min, 99% B. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Cogent Diamond Hydride (150 × 2.1mm,3.5um) |
| Chromatography Type: | Normal phase |
| Chromatography ID: | CH002025 |
| Chromatography Summary: | The mobile phase for RP separation consisted of 1 mM ammonium formate and 0.1% formic acid in H2O (solvent A) and 0.1% formic acid in CH3CN (solvent B). The gradient was as follows: 0–2 min, 1% B; 2– 20 min, to 50% B; 20–25 min, to 85% B; 25-30 min, to 99% B; 30.01-36 min, 1% B. |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent Zorbax RRHD SB-C18 (100 x 2.1mm,1.8um) |
| Flow Gradient: | 0-2 min, 1% B; 2- 20 min, to 50% B; 20-25 min, to 85% B; 25-30 min, to 99% B; 30.01-36 min, 1% B |
| Solvent A: | 100% water; 0.1% formic acid; 1 mM ammonium formate |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
