Summary of Study ST001767
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001767 |
Study Title | Application of the redox metabolite detection method for mammalian tissues (part II) |
Study Summary | This study was aimed at optimizing redox metabolites detection from mammalian tissues. Three different chromatographic conditions were compared as well as three different extraction conditions. This study was run on Accucore Amide HILIC chromatography |
Institute | Boston Children's Hospital, Harvard Medical School |
Department | Pathology |
Laboratory | Naama Kanarek |
Last Name | Petrova |
First Name | Boryana |
Address | 300 Longwood Av, Boston, MA, 2115, USA |
boryana.petrova@childrens.harvard.edu | |
Phone | 6173557433 |
Submit Date | 2021-04-22 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-05-17 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN002874 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Thermo Accucore 150 Amide HILIC (150x3mm,2.6um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | ppm |
Chromatography:
Chromatography ID: | CH002129 |
Chromatography Summary: | Accucore-HILIC chromatography: One ml of reconstituted sample was injected into a Thermo Fisher Scientific™ Accucore™ 150 Amide HILIC (150x3 mm, 2.6 mm particle size; Thermo Fisher Scientific) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific, San Jose, CA). Chromatographic separation was achieved using the following conditions: buffer A was acetonitrile; buffer B was 20 mM ammonium carbonate, 0.1% ammonium hydroxide. Gradient conditions were: linear gradient from 20% to 80% B; 20–20.5 min: from 80% to 20% B; 20.5–28 min: hold at 20% B. The column oven and autosampler tray were held at 35 °C and 4 °C, respectively. |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Accucore 150 Amide HILIC (150x3mm,2.6um) |
Column Temperature: | 35 |
Flow Gradient: | linear gradient from 20% to 80% B; 20-20.5 min: from 80% to 20% B; 20.5-28 min: hold at 20% B. |
Solvent A: | 100% acetonitrile |
Solvent B: | 100% water; 20 mM ammonium carbonate; 0.1% ammonium hydroxide |
Chromatography Type: | HILIC |