Summary of Study ST001794

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001138. The data can be accessed directly via it's Project DOI: 10.21228/M8M68K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001794
Study TitleMetabolomics Analysis of Time-Series Gastrointestinal Lumen Samples
Study SummarySamples were retrieved from a human small intestine samples over 8 hours. Metabolomics analysis followed resulting in many annotated metabolites. Intensity profiles gives insight into gastrointestinal functions.
Institute
University of California, Davis
Last NameFolz
First NameJacob
Address451 Health Science Drive
Emailjfolz@ucdavis.edu
Phone7155636311
Submit Date2021-05-17
Total Subjects1
Num Males1
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-06-01
Release Version1
Jacob Folz Jacob Folz
https://dx.doi.org/10.21228/M8M68K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002911 AN002912 AN002913 AN002914
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Vanquish Thermo Vanquish
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um) Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak Height Intensity Peak Height Intensity Peak Height Intensity Peak Height Intensity

Chromatography:

Chromatography ID:CH002157
Chromatography Summary:HILIC
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:The gradient started at 100% B, was held at 100% B from 0-2 minutes, decreased to 70% B between 2-7.7 minutes, decreased to 40% between 7.7 and 9.5 minutes, was held at 40% B from 9.5-12.75 minutes, returned to 100% B between 12.75-12.85 minutes, and was held at 100% B from 12.85-17 minutes.
Flow Rate:0.4 ml/min
Sample Injection:3 uL
Solvent A:100% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:95% acetonitrile/5% water; 0.1% formic acid; 10 mM ammonium formate
Chromatography Type:HILIC
  
Chromatography ID:CH002158
Chromatography Summary:CSH
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:65
Flow Gradient:The LC gradient started at 15% B, increased to 30% B from 0-2 minutes, increased to 48% B from 2-2.5 minutes, increased to 82% B from 2.5-11 minutes, increased to 99% B from 11-11.5 minutes, held at 99% B from 11.5-12 minutes, returned to 15% B from 12-12.1 minutes and held at 15% B from 12.1-15 minutes.
Flow Rate:0.6 ml/min
Sample Injection:4 uL
Solvent A:40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate(+ mode); 40% water/60% acetonitrile; 10 mM ammonium acetate(- mode)
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium formate for positive mode ionization, and 10mM ammonium acetate for negative mode ionization.
Chromatography Type:Reversed phase
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