Summary of Study ST001815
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001148. The data can be accessed directly via it's Project DOI: 10.21228/M89Q5T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001815 |
| Study Title | Metabolic Markers of Methotrexate Response in Juvenile Idiopathic Arthritis |
| Study Type | Clinical |
| Study Summary | Plasma from children with juvenile idiopathic arthritis collected pre-treatment and following 3 months of treatment with methotrexate were submitted for metabolomic profiling to the NIH West Coast Metabolomics Center. |
| Institute | University of Kansas |
| Department | Pharmacy Practice |
| Laboratory | Funk Lab |
| Last Name | funk |
| First Name | ryan |
| Address | 2106 Olathe Boulevard |
| ryanfunk@kumc.edu | |
| Phone | 9135885000 |
| Submit Date | 2021-05-18 |
| Num Groups | 1 |
| Total Subjects | 30 |
| Num Males | 9 |
| Num Females | 21 |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2021-06-17 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002944 | AN002945 | AN002946 | AN002947 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | GC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Leco Pegasus IV GC | Agilent 6530 | Agilent 6530 | Agilent 6530 |
| Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | EI | ESI | ESI | ESI |
| MS instrument type | GC-TOF | QTOF | QTOF | QTOF |
| MS instrument name | Leco Pegasus IV TOF | Agilent 6530 QTOF | Agilent 6530 QTOF | Agilent 6550 QTOF |
| Ion Mode | NEGATIVE | POSITIVE | POSITIVE | NEGATIVE |
| Units | Peak Height Intensity | Peak Height Intensity | Peak Height Intensity | Peak Height Intensity |
Chromatography:
| Chromatography ID: | CH002180 |
| Chromatography Summary: | Primary Metabolism |
| Instrument Name: | Leco Pegasus IV GC |
| Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
| Chromatography Type: | GC |
| Chromatography ID: | CH002181 |
| Chromatography Summary: | Biogenic Amines |
| Instrument Name: | Agilent 6530 |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002182 |
| Chromatography Summary: | Lipidomics |
| Instrument Name: | Agilent 6530 |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
