Summary of Study ST001855
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001170. The data can be accessed directly via it's Project DOI: 10.21228/M8G98C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001855 |
| Study Title | The metabolomic resetting effect of FG4592 in AKI to CKD transition (Part 1) |
| Study Summary | C57BL/6 mice were anesthetized using isoflurane. UIR was induced by clamping the right renal pedicle for 45 minutes and then releasing it to allow reperfusion, leaving the left kidney intact. Sham treated mice served as controls. Each mouse was located supine on a thermostatic pad (37 °C) to maintain its body temperature throughout the whole process. After 3 days of recovery, the mice received a daily intraperitoneal (i.p.) injection of FG4592 (10 mg/kg) or vehicle for 18 consecutive days. After treatment, the mice were sacrificed. Non-target metabolomics analysis was carried out using the kidney tissues of mice sacrificed at day 21 after UIR. |
| Institute | Children's Hospital of Nanjing Medical University |
| Last Name | Chen |
| First Name | Weiyi |
| Address | 72 Guangzhou Road, Nanjing 210008, P. R. of China |
| chen.weiyi@qq.com | |
| Phone | 0086-25-8311-7309 |
| Submit Date | 2021-06-30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-07-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003006 | AN003007 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Ultimate 3000 system | Thermo Ultimate 3000 system |
| Column | Waters ACQUITY UPLC HSS T3 | Waters ACQUITY UPLC HSS T3 |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | AUC | AUC |
Chromatography:
| Chromatography ID: | CH002229 |
| Chromatography Summary: | A 2 μL aliquot of the samples was injected into a Thermo Ultimate 3000 system equipped with an ACQUITY UPLC® HSS T3 column (150 × 2.1 mm, 1.8 μm, Waters, Milford, MA, USA) maintained at 40 °C. The mobile phase for positive mode analysis consists of 0.1% formic acid in water (phase C) and 0.1% formic acid in acetonitrile (phase D), while the mobile phase for negative mode analysis consists of 5 mM ammonium formate in water (phase A) and acetonitrile (phase B). Phase C and D, as well as phase A and B, were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0~1 min; 2% B/D; 1~9 min, 2%~50% B/D; 9~12 min, 50%~98% B/D; 12~13.5 min, 98% B/D; 13.5~14 min, 98%~2% B/D; 14~20 min, 2% D-positive model (14~17 min, 2% B-negative model). |
| Instrument Name: | Thermo Ultimate 3000 system |
| Column Name: | Waters ACQUITY UPLC HSS T3 |
| Column Temperature: | 40 |
| Flow Gradient: | phase A and B were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0-1 min; 2% A/B; 1-9 min, 2%-50% A/B; 9-12 min, 50%-98% A/B; 12-13.5 min, 98% A/B; 13.5-14 min, 98%-2% A/B; 14-20 min, 2% B-positive model (14-17 min, 2% B-negative model) |
| Flow Rate: | 0.25ml/min |
| Solvent A: | 100% water; 5mM ammonium formate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
