Summary of Study ST001856

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001170. The data can be accessed directly via it's Project DOI: 10.21228/M8G98C This work is supported by NIH grant, U2C- DK119886.

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Study IDST001856
Study TitleThe metabolomic resetting effect of FG4592 in AKI to CKD transition (Part 2)
Study SummaryC57BL/6 mice were anesthetized using isoflurane. UIR was induced by clamping the right renal pedicle for 45 minutes and then releasing it to allow reperfusion, leaving the left kidney intact. Sham treated mice served as controls. Each mouse was located supine on a thermostatic pad (37 °C) to maintain its body temperature throughout the whole process. After 3 days of recovery, the mice received a daily intraperitoneal (i.p.) injection of FG4592 (10 mg/kg) or vehicle for 7 consecutive days. After treatment, the mice were sacrificed.Non-target metabolomics analysis was carried out using the kidney tissues of mice sacrificed at day 10 after UIR.
Institute
Children's Hospital of Nanjing Medical University
Last NameWeiyi
First NameChen
Address72 Guangzhou Road, Nanjing 210008, P. R. of China
Emailchen.weiyi@qq.com
Phone+862583117309
Submit Date2021-07-01
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-07-07
Release Version1
Chen Weiyi Chen Weiyi
https://dx.doi.org/10.21228/M8G98C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003008 AN003009
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Ultimate 3000 system Thermo Ultimate 3000 system
Column ACQUITY UPLC® HSS T3 column ACQUITY UPLC® HSS T3 column
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units AUC AUC

Chromatography:

Chromatography ID:CH002230
Chromatography Summary:A 2 μL aliquot of the samples was injected into a Thermo Ultimate 3000 system equipped with an ACQUITY UPLC® HSS T3 column (150 × 2.1 mm, 1.8 μm, Waters, Milford, MA, USA) maintained at 40 °C. The mobile phase for positive mode analysis consists of 0.1% formic acid in water (phase C) and 0.1% formic acid in acetonitrile (phase D), while the mobile phase for negative mode analysis consists of 5 mM ammonium formate in water (phase A) and acetonitrile (phase B). Phase C and D, as well as phase A and B, were mixed and delivered at a flow rate of 0.25 mL/min with a gradient program as follows: 0~1 min; 2% B/D; 1~9 min, 2%~50% B/D; 9~12 min, 50%~98% B/D; 12~13.5 min, 98% B/D; 13.5~14 min, 98%~2% B/D; 14~20 min, 2% D-positive model (14~17 min, 2% B-negative model).
Instrument Name:Thermo Ultimate 3000 system
Column Name:ACQUITY UPLC® HSS T3 column
Chromatography Type:Reversed phase
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