Summary of Study ST001927

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001217. The data can be accessed directly via it's Project DOI: 10.21228/M8D693 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001927
Study TitleFungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach.
Study TypeUntargeted Metabolomics
Study SummaryEntomopathogenic fungi have been successfully used to control agricultural pests. They infect insects by coming into direct contact with their cuticle or when feeding on contaminated leaves or fruits. After contact with the insect, the entomopathogenic fungus penetrates its body cavity, where it grows and colonizes it from within, causing its death The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. Despite recent developments and growing efforts to better understand fungal metabolism and metabolites, much remains unknown. Metabolomics therefore represents an important field for evaluating the metabolites produced or modified by an organism or its relationship with the environment. In the present study, we aim to use untargeted metabolomics with gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations caused by the co-cultivation of these strains and to correlate the metabolites produced by this consortium with the increased mortality in D. fovealis observed previosly.
Institute
Universidade Federal do Paraná
DepartmentPatologia Básica
LaboratoryLaboratório de Microbiologia e Biologia Molecular
Last NameStuart
First NameAndressa
AddressAv. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, Curitiba, Paraná, Brasil
Emailandressa.katiski@gmail.com
Phone5541991922779
Submit Date2021-09-29
Num Groups3
Total Subjects15
Study CommentsTwo genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The metabolomic analysis were performed at the Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). Three reads of every biological replicate (five per treatment) were performed, generating fifteen readings for each treatment. Pools of metabolites from each group were created as a quality control.
Raw Data AvailableYes
Raw Data File Type(s)cdf, raw(Waters)
Analysis Type DetailGC-MS/LC-MS
Release Date2022-10-03
Release Version1
Andressa Stuart Andressa Stuart
https://dx.doi.org/10.21228/M8D693
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003133 AN003134 AN003135
Analysis type MS MS MS
Chromatography type GC Reversed phase Reversed phase
Chromatography system Agilent 7890A Waters Acquity UPLC Waters Acquity UPLC
Column Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um)
MS Type EI ESI ESI
MS instrument type GC x GC-TOF QTOF QTOF
MS instrument name Leco Pegasus 4D GCxGC TOF Waters Ultima QTOF Waters Ultima QTOF
Ion Mode UNSPECIFIED POSITIVE NEGATIVE
Units Relative intensity Relative intensity

Chromatography:

Chromatography ID:CH002315
Chromatography Summary:(.cdf)
Methods Filename:LCMSMS
Instrument Name:Agilent 7890A
Column Name:Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um)
Column Temperature:70 - 320 ºC
Flow Rate:1 mL.min-1
Injection Temperature:280 ºC
Sample Injection:1 uL
Oven Temperature:70°C for 2 min, increasing by 15°C·min-1 until it reached 320°C and then held at this temperature for 4 min.
Chromatography Type:GC
  
Chromatography ID:CH002316
Chromatography Summary:Positive Mode (.raw)
Methods Filename:LCMSMS
Instrument Name:Waters Acquity UPLC
Column Name:Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um)
Column Temperature:35 ºC
Flow Gradient:95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min
Flow Rate:0.5 mL·min-1
Solvent A:Water; formic acid
Solvent B:100% acetonitrile; formic acid.
Chromatography Type:Reversed phase
  
Chromatography ID:CH002317
Chromatography Summary:Negative mode (.raw)
Methods Filename:LCMSMS
Instrument Name:Waters Acquity UPLC
Column Name:Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um)
Column Temperature:35 ºC
Flow Gradient:95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min
Flow Rate:0.5 mL·min-1
Solvent A:Water; formic acid
Solvent B:100% acetonitrile; formic acid.
Chromatography Type:Reversed phase
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