Summary of Study ST002236
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001426. The data can be accessed directly via it's Project DOI: 10.21228/M8D42R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002236 |
| Study Title | The impact of IgE in gut and serum metabolomes in a murine experimental model of allergic enteritis |
| Study Type | Case-control study |
| Study Summary | The pathological mechanism of the gastrointestinal forms of food allergies is less understood in comparison to other clinical phenotypes, such as asthma, and anaphylaxis, partly due to difficulty in the access to intestinal tissues and because of a highly complex interplay between microbiota and intestinal mucosa. Importantly, a high level of IgE is a poor prognostic factor in gastrointestinal allergies. This study aimed to investigate how IgE influences the development of intestinal inflammation and the metabolome in allergic enteritis (AE), using IgE knock-in (IgEki) mice expressing high levels of IgE. Ovalbumin-sensitized and egg-white diet fed (OVA/EW) BALB/c WT mice developed moderate AE, whereas OVA/EW IgEki mice induced more aggravated intestinal inflammation with enhanced eosinophil accumulation. |
| Institute | Institute of Applied Molecular Medicine |
| Last Name | Zubeldia-Varela |
| First Name | Elisa |
| Address | Avda. Monteprincipe s/n 28668 Boadilla del Monte, Madrid, España |
| elisa.zubeldiavarela@ceu.es | |
| Phone | Tlf: 91 372 47 00 ext. 14675 |
| Submit Date | 2022-07-19 |
| Num Groups | 4 groups: BALB/c wild type (WT) and IgE knock-in mice (C.Ighg1tm1.1Pyu) in the BALB/c background, both sensitised and non-sensitised to ovalbumin. |
| Total Subjects | 29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2022-08-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003646 | AN003647 | AN003648 | AN003649 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | CE | GC |
| Chromatography system | Agilent 1290 | Agilent 1290 | Agilent 7100 CE | Agilent 7890A |
| Column | Zorbax Extend C18 (50 x 2.1 mm,1.8um) | Zorbax Extend C18 (50 x 2.1 mm,1.8um) | Fused-silica capillary (total length,100 cm;,50um) | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| MS Type | ESI | ESI | ESI | EI |
| MS instrument type | QTOF | QTOF | TOF | Single quadrupole |
| MS instrument name | Agilent 6545 QTOF | Agilent 6545 QTOF | Agilent 6224 TOF | Agilent 5975C |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | POSITIVE |
| Units | Peak Area | peak area | Peak Area | Peak area |
Chromatography:
| Chromatography ID: | CH002700 |
| Chromatography Summary: | For LC-MS, 0.5 µL of sample were injected into a Zorbax Extend C18 (50 x 2.1 mm, 1.8 µm) maintained at 60 °C. The flow rate was set at 0.6 mL/min. . The flow rate was set at 0.6 mL/min. The elution gradient involved a mobile phase consisting of: (A) 0.1% formic acid (FA) in water and (B) 0.1% FA in acetonitrile (ACN). The gradient was 5% B (0–1 min), 5 to 80% B (1–7 min), 80 to 100% B (7–11.5 min), and 100 to 5% B (11.5–12 min). The system was finally held at 5% B for 3 min to re-equilibrate the system (15 min of total analysis time). The ESI data were acquired in both modes, ESI (+) and ESI (-), in separate runs. The capillary voltage was set at 3,000V for ESI (+) and 4,000V for ESI (-). The drying gas flow rate was 12 L/min at 250 °C and gas nebulizer at 52 psi; nozzle voltage was 1000V; fragmentor voltage was 175 V; skimmer and octopole radio frequency voltage (OCT RF Vpp) were set to 65 and 750 V, respectively. Data were collected in the centroid mode at a scan rate of 1.5 spectra per second. |
| Methods Filename: | Protocolmethods.docx |
| Instrument Name: | Agilent 1290 |
| Column Name: | Zorbax Extend C18 (50 x 2.1 mm,1.8um) |
| Column Temperature: | 60 |
| Flow Gradient: | 5% B (0-1 min), 5 to 80% B (1-7 min), 80 to 100% B (7-11.5 min), and 100 to 5% B (11.5-12 min). The system was finally held at 5% B for 3 min to re-equilibrate the system (15 min of total analysis time). |
| Flow Rate: | 0.6 mL/min |
| Sample Injection: | 0.5 µL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002701 |
| Chromatography Summary: | For LC-MS, 0.5 µL of sample were injected into a Zorbax Extend C18 (50 x 2.1 mm, 1.8 µm) maintained at 60 °C. The flow rate was set at 0.6 mL/min. . The flow rate was set at 0.6 mL/min. The elution gradient involved a mobile phase consisting of: (A) 0.1% formic acid (FA) in water and (B) 0.1% FA in acetonitrile (ACN). The gradient was 5% B (0–1 min), 5 to 80% B (1–7 min), 80 to 100% B (7–11.5 min), and 100 to 5% B (11.5–12 min). The system was finally held at 5% B for 3 min to re-equilibrate the system (15 min of total analysis time). The ESI data were acquired in both modes, ESI (+) and ESI (-), in separate runs. The capillary voltage was set at 3,000V for ESI (+) and 4,000V for ESI (-). The drying gas flow rate was 12 L/min at 250 °C and gas nebulizer at 52 psi; nozzle voltage was 1000V; fragmentor voltage was 175 V; skimmer and octopole radio frequency voltage (OCT RF Vpp) were set to 65 and 750 V, respectively. Data were collected in the centroid mode at a scan rate of 1.5 spectra per second. |
| Methods Filename: | Protocolmethods.docx |
| Instrument Name: | Agilent 1290 |
| Column Name: | Zorbax Extend C18 (50 x 2.1 mm,1.8um) |
| Column Temperature: | 60 |
| Flow Gradient: | 5% B (0-1 min), 5 to 80% B (1-7 min), 80 to 100% B (7-11.5 min), and 100 to 5% B (11.5-12 min). The system was finally held at 5% B for 3 min to re-equilibrate the system (15 min of total analysis time). |
| Flow Rate: | 0.6 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002702 |
| Chromatography Summary: | For CE-MS, the separation occurred in a fused-silica capillary (Agilent: total length, 100 cm; i.d., 50 µm). All separations were performed in normal polarity with a background electrolyte containing 1.0 M formic acid in 10% MeOH (v/v) at 20°C. New capillaries were preconditioned with a flush of 1.0 M NaOH for 30 min, followed by MilliQ water for 30 min and the background electrolyte for 30 min. Before each analysis, the capillary was conditioned with a flush of background electrolyte for 5 min. The sheath liquid (10 µL/min) was MeOH:H2O (1:1) containing 1.0 mM FA with two reference masses 121.0509 (purine), and 922.0098 (HP), which allowed for correction and higher mass accuracy in the MS. The sheath liquid was infused by an ISO Pump (1200 Agilent). The samples were hydrodynamically injected at 50 mBar for 50 s. The stacking was performed by applying the background electrolyte at 100 mBar for 20 s. The separation voltage was 30 kV, the internal pressure was 25 mBar and the analyses were performed in 40 min. |
| Methods Filename: | Protocolmethods.docx |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | Fused-silica capillary (total length,100 cm;,50um) |
| Chromatography Type: | CE |
| Chromatography ID: | CH002703 |
| Chromatography Summary: | For GC-MS, two microliters (2 µL) of the derivatized sample were injected through a DB5-MS GC-Column (30 m length, 0.25 mm i.d., 0.25 µm film 95% dimethyl/5% diphenylpolysiloxane) with an integrated precolumn (10 m J&W, Agilent). Carrier gas (Helium) flow rate was set at 1 mL/min and injector temperature at 250 °C. Split ratio was fixed from 1:5 to 1:10 with 3 to 10 mL/min Helium split flow into a Restek 20782 (Bellefonte, PA, USA) deactivated glass-wool split liner. The temperature gradient was programmed as follows: the initial oven temperature was set at 60 °C (held for 1 min), increased to 325 °C at 10 °C/min rate (within 26.5 min) and was held at 325 °C for 10 min. The total run time was 37.5 min. A cool-down period of 10 min was applied before the next injection. |
| Methods Filename: | Protocolmethods.docx |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Chromatography Type: | GC |
