Summary of Study ST002281
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001461. The data can be accessed directly via it's Project DOI: 10.21228/M8W122 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002281 |
Study Title | Metabolite patterns between isogenic normal hiPSCs and Trisomy hiPSC |
Study Summary | We wanted to compare metabolites patterns between isogenic normal hiPSCs and Trisomy hiPSCs. All hiPSCs cell lines were generated from same mosaic Down symdrome patient. |
Institute | Guangdong Provincial People's Hospital |
Department | Medical Research Center |
Laboratory | Liu lab |
Last Name | Liu |
First Name | Juli |
Address | Zhongshan 2nd road |
liujuli@gdph.org.cn | |
Phone | +8602083827812 |
Submit Date | 2022-09-09 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | APCI-MS |
Release Date | 2024-09-13 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003725 | AN003726 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Hypesil Gold | Hypesil Gold |
MS Type | APCI | APCI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive HF-X Orbitrap | Thermo Q Exactive HF-X Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | pmoles/l | pmoles/l |
Chromatography:
Chromatography ID: | CH002759 |
Chromatography Summary: | UHPLC-MS/MS analyses were performed using a Vanquish UHPLC system (ThermoFisher, Germany) coupled with an Orbitrap Q ExactiveTMHF-X mass spectrometer (Thermo Fisher,Germany) in Novogene Co., Ltd. (Beijing, China). Samples were injected onto a Hypesil Gold column (100×2.1 mm, 1.9µm) using a 17-min linear gradient at a flow rate of 0.2mL/min. The eluents for the positive polarity mode were eluent A (0.1% FA in Water) and eluent B (Methanol).The eluents for the negative polarity mode were eluent A (5 mMammonium acetate, pH 9.0) and eluent B (Methanol).The solvent gradient was set as follows: 2% B, 1.5 min; 2-100% B, 3 min; 100% B, 10 min;100-2% B, 10.1 min;2% B, 12 min. Q ExactiveTM HF-X mass spectrometer was operated in positive/negative polarity mode with spray voltage of 3.5 kV, capillary temperature of 320°C, sheath gas flow rate of 35 psi and aux gas flow rate of 10 L/min, S-lens RF level of 60, Aux gas heater temperature of 350°C. |
Instrument Name: | Thermo Vanquish |
Column Name: | Hypesil Gold |
Flow Gradient: | 2% B, 1.5 min; 2-100% B, 3 min; 100% B, 10 min;100-2% B, 10.1 min;2% B, 12 min |
Flow Rate: | 0.2mL/min |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.0 |
Solvent B: | 100% methanol |
Chromatography Type: | Reversed phase |