Summary of Study ST002281

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001461. The data can be accessed directly via it's Project DOI: 10.21228/M8W122 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002281
Study TitleMetabolite patterns between isogenic normal hiPSCs and Trisomy hiPSC
Study SummaryWe wanted to compare metabolites patterns between isogenic normal hiPSCs and Trisomy hiPSCs. All hiPSCs cell lines were generated from same mosaic Down symdrome patient.
Institute
Guangdong Provincial People's Hospital
DepartmentMedical Research Center
LaboratoryLiu lab
Last NameLiu
First NameJuli
AddressZhongshan 2nd road
Emailliujuli@gdph.org.cn
Phone+8602083827812
Submit Date2022-09-09
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailAPCI-MS
Release Date2024-09-13
Release Version1
Juli Liu Juli Liu
https://dx.doi.org/10.21228/M8W122
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003725 AN003726
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Hypesil Gold Hypesil Gold
MS Type APCI APCI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap Thermo Q Exactive HF-X Orbitrap
Ion Mode POSITIVE NEGATIVE
Units pmoles/l pmoles/l

Chromatography:

Chromatography ID:CH002759
Chromatography Summary:UHPLC-MS/MS analyses were performed using a Vanquish UHPLC system (ThermoFisher, Germany) coupled with an Orbitrap Q ExactiveTMHF-X mass spectrometer (Thermo Fisher,Germany) in Novogene Co., Ltd. (Beijing, China). Samples were injected onto a Hypesil Gold column (100×2.1 mm, 1.9µm) using a 17-min linear gradient at a flow rate of 0.2mL/min. The eluents for the positive polarity mode were eluent A (0.1% FA in Water) and eluent B (Methanol).The eluents for the negative polarity mode were eluent A (5 mMammonium acetate, pH 9.0) and eluent B (Methanol).The solvent gradient was set as follows: 2% B, 1.5 min; 2-100% B, 3 min; 100% B, 10 min;100-2% B, 10.1 min;2% B, 12 min. Q ExactiveTM HF-X mass spectrometer was operated in positive/negative polarity mode with spray voltage of 3.5 kV, capillary temperature of 320°C, sheath gas flow rate of 35 psi and aux gas flow rate of 10 L/min, S-lens RF level of 60, Aux gas heater temperature of 350°C.
Instrument Name:Thermo Vanquish
Column Name:Hypesil Gold
Flow Gradient:2% B, 1.5 min; 2-100% B, 3 min; 100% B, 10 min;100-2% B, 10.1 min;2% B, 12 min
Flow Rate:0.2mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.0
Solvent B:100% methanol
Chromatography Type:Reversed phase
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