Summary of Study ST002327
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001491. The data can be accessed directly via it's Project DOI: 10.21228/M80M7R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002327 |
Study Title | Effect of PARK7 gene KO on midbrain organoids |
Study Summary | DJ1 KO was generated in BJsips iPSC and differentiated into midbrain organoids with the respective iPSC controls. The midbrain organoids were collected at day 40, 100 and 200 after differentiation. |
Institute | Icahn School of Medicine at Mount Sinai |
Last Name | Morrone Parfitt |
First Name | Gustavo |
Address | One Gustave L. Levy Place, Box 1065 |
gustavo.parfitt@mssm.edu | |
Phone | 212-659-5993 |
Submit Date | 2022-09-06 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-11-18 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003796 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Dionex Ultimate 3000TM |
Column | Millipore(TM) ZIC-pHILIC |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | POSITIVE |
Units | intensity |
Chromatography:
Chromatography ID: | CH002809 |
Chromatography Summary: | The LC column was a Millipore TMZIC-pHILIC (2.1x150 mm, 5μm) coupled to a Dionex Ultimate 3000TM system and the column oven temperature was set to 25oC for the gradient elution. A flow rate of 100μL/min was used with the 10 mM ammonium carbonate in water (A), pH 9.0, and acetonitrile (B). The gradient profile was 80-20% B (0-30min), 20-80% B (30-31min), 80-80% B (31-42min). Injection volume was set to 2 μL for all analyses (42min total run time per injection). |
Methods Filename: | metabolomics_methods.pdf |
Instrument Name: | Dionex Ultimate 3000TM |
Column Name: | Millipore(TM) ZIC-pHILIC |
Column Temperature: | 25 |
Flow Gradient: | 80-20% B (0-30min), 20-80% B (30-31min), 80-80% B (31-42min). |
Flow Rate: | 100µL/min |
Solvent A: | 100% water; 10 mM ammonium carbonate, pH 9.0 |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |