Summary of Study ST002327

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001491. The data can be accessed directly via it's Project DOI: 10.21228/M80M7R This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002327
Study TitleEffect of PARK7 gene KO on midbrain organoids
Study SummaryDJ1 KO was generated in BJsips iPSC and differentiated into midbrain organoids with the respective iPSC controls. The midbrain organoids were collected at day 40, 100 and 200 after differentiation.
Institute
Icahn School of Medicine at Mount Sinai
Last NameMorrone Parfitt
First NameGustavo
AddressOne Gustave L. Levy Place, Box 1065
Emailgustavo.parfitt@mssm.edu
Phone212-659-5993
Submit Date2022-09-06
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-11-18
Release Version1
Gustavo Morrone Parfitt Gustavo Morrone Parfitt
https://dx.doi.org/10.21228/M80M7R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003796
Analysis type MS
Chromatography type HILIC
Chromatography system Dionex Ultimate 3000TM
Column Millipore(TM) ZIC-pHILIC
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE
Units intensity

Chromatography:

Chromatography ID:CH002809
Chromatography Summary:The LC column was a Millipore TMZIC-pHILIC (2.1x150 mm, 5μm) coupled to a Dionex Ultimate 3000TM system and the column oven temperature was set to 25oC for the gradient elution. A flow rate of 100μL/min was used with the 10 mM ammonium carbonate in water (A), pH 9.0, and acetonitrile (B). The gradient profile was 80-20% B (0-30min), 20-80% B (30-31min), 80-80% B (31-42min). Injection volume was set to 2 μL for all analyses (42min total run time per injection).
Methods Filename:metabolomics_methods.pdf
Instrument Name:Dionex Ultimate 3000TM
Column Name:Millipore(TM) ZIC-pHILIC
Column Temperature:25
Flow Gradient:80-20% B (0-30min), 20-80% B (30-31min), 80-80% B (31-42min).
Flow Rate:100µL/min
Solvent A:100% water; 10 mM ammonium carbonate, pH 9.0
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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