Summary of Study ST002343

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001502. The data can be accessed directly via it's Project DOI: 10.21228/M8JD81 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002343
Study TitleThe impact of myriocin and dietary serine restriction on paw skin sphingolipid diversity
Study SummaryWe analyzed paw skin polar and lipid metabolites in mice (C57BL/6J) in response to two dietary interventions. The first was: 1) low fat (LFD), 2) serine/glycine-free LFD (-SG LFD), high fat (HFD), and serine/glycine-free HFD (-SG HFD). And the second was: 1) low fat (LFD), high fat (HFD), serine/glycine-free HFD (-SG HFD), and serine/glycine-free HFD (-SG HFD) with myriocin (0.3 mg/kg every other day) treatment. The goal of the study was to determine the impact of dietary serine/glycine restriction and myriocin treatment on the hepatic lipidome.
Institute
Salk Institute for Biological Studies
DepartmentMolecular and Cell Biology Laboratory
LaboratoryMetallo Lab
Last NameHandzlik
First NameMichal
Address10010 N Torrey Pines Rd, La Jolla, California, 92037, USA
Emailmhandzlik@salk.edu
Phone2069601945
Submit Date2022-11-11
Num GroupsLFD, -SG LFD, HFD, -SG HFD, LFD+Veh, HFD+Veh, -SG HFD+Veh, -SG HFD+Myr
Total Subjects47
Num Males47
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2024-01-01
Release Version1
Michal Handzlik Michal Handzlik
https://dx.doi.org/10.21228/M8JD81
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003827
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 6460C
Column Spectra 3 μm C8SR 150 × 3 mm inner diameter, Peeke Scientific
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6460 QQQ
Ion Mode POSITIVE
Units normalized ion count/mg protein

Chromatography:

Chromatography ID:CH002832
Chromatography Summary:The gradient elution program consisted of the following profile: 0 min, 82% B; 3 min, 82% B; 4 min, 90% B, 18 min, 99% B; 25 min, 99%, 27 min, 82% B, 30 min, 82% B.
Instrument Name:Agilent 6460C
Column Name:Spectra 3 μm C8SR 150 × 3 mm inner diameter, Peeke Scientific
Column Temperature:40
Flow Rate:0.5 ml/min
Solvent A:100% water; 0.2% formic acid; 2 mM ammonium formate
Solvent B:100% methanol; 0.2% formic acid; 1 mM ammonium formate
Chromatography Type:Reversed phase
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