Summary of Study ST002343
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001502. The data can be accessed directly via it's Project DOI: 10.21228/M8JD81 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002343 |
Study Title | The impact of myriocin and dietary serine restriction on paw skin sphingolipid diversity |
Study Summary | We analyzed paw skin polar and lipid metabolites in mice (C57BL/6J) in response to two dietary interventions. The first was: 1) low fat (LFD), 2) serine/glycine-free LFD (-SG LFD), high fat (HFD), and serine/glycine-free HFD (-SG HFD). And the second was: 1) low fat (LFD), high fat (HFD), serine/glycine-free HFD (-SG HFD), and serine/glycine-free HFD (-SG HFD) with myriocin (0.3 mg/kg every other day) treatment. The goal of the study was to determine the impact of dietary serine/glycine restriction and myriocin treatment on the hepatic lipidome. |
Institute | Salk Institute for Biological Studies |
Department | Molecular and Cell Biology Laboratory |
Laboratory | Metallo Lab |
Last Name | Handzlik |
First Name | Michal |
Address | 10010 N Torrey Pines Rd, La Jolla, California, 92037, USA |
mhandzlik@salk.edu | |
Phone | 2069601945 |
Submit Date | 2022-11-11 |
Num Groups | LFD, -SG LFD, HFD, -SG HFD, LFD+Veh, HFD+Veh, -SG HFD+Veh, -SG HFD+Myr |
Total Subjects | 47 |
Num Males | 47 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2024-01-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN003827 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 6460C |
Column | Spectra 3 μm C8SR 150 × 3 mm inner diameter, Peeke Scientific |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6460 QQQ |
Ion Mode | POSITIVE |
Units | normalized ion count/mg protein |
Chromatography:
Chromatography ID: | CH002832 |
Chromatography Summary: | The gradient elution program consisted of the following profile: 0 min, 82% B; 3 min, 82% B; 4 min, 90% B, 18 min, 99% B; 25 min, 99%, 27 min, 82% B, 30 min, 82% B. |
Instrument Name: | Agilent 6460C |
Column Name: | Spectra 3 μm C8SR 150 × 3 mm inner diameter, Peeke Scientific |
Column Temperature: | 40 |
Flow Rate: | 0.5 ml/min |
Solvent A: | 100% water; 0.2% formic acid; 2 mM ammonium formate |
Solvent B: | 100% methanol; 0.2% formic acid; 1 mM ammonium formate |
Chromatography Type: | Reversed phase |