Summary of Study ST002465
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001468. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT5R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002465 |
| Study Title | Map of microbially induced metabolic changes across diverse body sites in mice - Mouse Data |
| Study Summary | Tissue samples from contents along the intestinal tract and systemic sites in mice that did not have any bacteria (germ free) or colonized with a simplified microbiota or more complex microbiota. |
| Institute | University of Calgary |
| Department | Physiology and Pharmacology |
| Laboratory | McCoy |
| Last Name | Brown |
| First Name | Kirsty |
| Address | 3330 Hospital Dr NW |
| kirsty.brown12@gmail.com; kathy.mccoy@ucalgary.ca | |
| Phone | 2508692232 |
| Submit Date | 2023-02-04 |
| Total Subjects | 72 |
| Num Males | 36 |
| Num Females | 36 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004021 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | Thermo Syncronis HILIC (100 x 2.1mm, 1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | NEGATIVE |
| Units | AUC |
Chromatography:
| Chromatography ID: | CH002971 |
| Chromatography Summary: | All metabolomics data were collected at the Calgary Metabolomics Research Facility (CMRF). Briefly, samples were centrifuged (20,817 xg, 15 minutes, 4°C), then 200µL was transferred to a deep-well 96-well plate (Thermo FisherF) for LC-MS analysis. Data were collected on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (Thermo-Fisher) coupled to a Vanquish™ UHPLC System (Thermo-Fisher). Metabolites were chromatographically separated on Syncronis HILIC UHPLC column (2.1mm x 100mm x 1.7um, Thermo-Fisher) at the flow rate of 600uL/min using a binary solvent system (solvent A, 20mM ammonium formate pH 3.0 in MS grade H20 and solvent B, MS grade acetonitrile with 0.1% formic acid (%v/v)) and the following gradient: 0-2 mins, 100 %B; 2-7 mins, 100-80 %B; 7-10 mins, 80-5 %B; 10-12 mins, 5% B; 12-13 mins, 5-100 %B; 13-15 mins, 100 %B. The samples injection volume was 2uL. MassM data were acquired in MS1, negative full scan mode at a resolution of 240,000 scanning from 50-750m/z. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Syncronis HILIC (100 x 2.1mm, 1.7um) |
| Column Temperature: | 30C |
| Flow Gradient: | 2 stage linear gradient |
| Flow Rate: | 600uL/min |
| Sample Injection: | 2uL |
| Solvent A: | 100% water; 20mM ammonium formate (pH3) |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 15 minutes |
| Washing Buffer: | 10% MeOH in H2O + 0.1% formic acid |
| Target Sample Temperature: | 4 |
| Chromatography Type: | HILIC |
