Summary of Study ST002475
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001598. The data can be accessed directly via it's Project DOI: 10.21228/M84Q58 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002475 |
| Study Title | Psychobiotic Lactobacillus plantarum JYLP-326 relieves anxiety, depression, and insomnia symptoms in test anxious college students via modulating the gut microbiota and its metabolism |
| Study Summary | Test anxiety frequently occurs in college students and harms their physical and psychological health, but suitable interventions or therapeutical strategies are still missing. The present study aims to evaluate the potential effects of Lactobacillus plantarum JYLP-326 on test anxious college students. Sixty anxious students were enrolled and randomly allocated to the placebo group and the probiotic group, which were instructed to take placebo and JYLP-326 products twice per day for three weeks, respectively. Thirty unanxious students with no treatments were assigned to a regular control group. The anxiety, depression, and insomnia questionnaires were used to measure students’ mental states at the baseline and the end of this study. 16S rRNA sequencing and untargeted metabolomics were performed to analyze the changes in the gut microbiota and fecal metabolism. The questionnaire results suggested that JYLP-326 administration could relieve the symptoms of anxiety, depression, and insomnia in test anxious students. The gut microbiomes of the placebo group showed a significantly greater α diversity index than the control group (p < 0.05). An increased abundance of Bacteroides and Roseburia was observed in the placebo group, and the relative abundance of Prevotella and Bifidobacterium decreased. Whereas JYLP-326 administration could partly restore the disturbed gut microbiota. Additionally, test anxiety was correlation with disordered fecal metabolomics such as a higher Ethyl sulfate and a lower Cyclohexylamine, which could be reversed after taking JYLP-326. Furthermore, the changed microbiota and fecal metabolites were significantly associated with anxiety-related symptoms. These results indicated that the intervention of L. plantarum JYLP-326 could be an effective strategy to alleviate anxiety, depression and insomnia in test anxious college students. |
| Institute | Nanchang university |
| Last Name | Ruizhe |
| First Name | Zhu |
| Address | National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, Nanchang, China |
| arthurzhu233@outlook.com | |
| Phone | 18779116254 |
| Submit Date | 2023-02-10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-16 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004042 | AN004043 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple quadrupole |
| MS instrument name | ABI Sciex 6600 TripleTOF | ABI Sciex 6600 TripleTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Original peak intensity values | Original peak intensity values |
Chromatography:
| Chromatography ID: | CH002990 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.7um) |
| Column Temperature: | 25 |
| Flow Gradient: | The gradient elution program was as follows: 0 --- 0.5 min, 95% B; 0.5 --- 7 min, B varied linearly from 95% to 65 %; 7 --- 8 min, B varied linearly from 65% to 40%; 8---9 min, B was maintained at 40%; 9---9.1 min, B varied linearly from 40% to 95%; 9.1--12 min, B maintained at 95%. |
| Flow Rate: | 0.5 mL/min |
| Solvent A: | 100% water; 25 mM ammonium acetate; 25 mM ammonia |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
