Summary of Study ST002479

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001599. The data can be accessed directly via it's Project DOI: 10.21228/M8113P This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002479
Study TitleHigh body temperature increases gut microbiota-dependent host resistance to influenza A virus and SARS-CoV-2 infection (Hamster)
Study SummaryWhile a common symptom of influenza and coronavirus disease 2019 (COVID-19) is fever, its physiological role on host resistance to viral infection remains less clear. Here, we demonstrate that exposure of mice to the high ambient temperature of 36 °C increase host resistance to viral pathogens including influenza virus and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). High heat-exposed mice increase basal body temperature over 38 °C to enable more bile acids production in a gut microbiota-dependent manner. The gut microbiota-derived deoxycholic acid (DCA) and its plasma membrane-bound receptor Takeda G-protein-coupled receptor 5 (TGR5) signaling increase host resistance to influenza virus infection by suppressing virus replication and neutrophil-dependent tissue damage. Furthermore, the DCA and its nuclear farnesoid X receptor (FXR) agonist protect Syrian hamster from lethal SARS-CoV-2 infection. Moreover, we demonstrate that certain bile acids are reduced in the plasma of COVID-19 patients who developed moderate I/II disease compared with minor illness group. These findings uncover an unexpected mechanism by which virus-induced high fever increases host resistance to influenza virus and SARS-CoV-2 in a gut microbiota-dependent manner.
Keio University
Last NameFukuda
First NameShinji
AddressKakuganji 246-2, Mizukami, Tsuruoka City Yamagata,Japan
Submit Date2023-02-14
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailCE-MS
Release Date2023-03-10
Release Version2
Shinji Fukuda Shinji Fukuda application/zip

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Combined analysis:

Analysis ID AN004079 AN004080
Analysis type MS MS
Chromatography type CE CE
Chromatography system Agilent CE Agilent CE
Column Fused-silica, i.d. 50 µm(cation) COSMO(+), i.d.50 μm(anion)
MS instrument type Other Other
MS instrument name Agilent CE-TOFMS Agilent CE-TOFMS
Units nmol/g nmol/g


Chromatography ID:CH003021
Instrument Name:Agilent CE
Column Name:Fused-silica, i.d. 50 µm(cation)
Column Temperature:none
Flow Gradient:none
Flow Rate:none
Solvent A:none
Solvent B:none
Chromatography Type:CE
Chromatography ID:CH003022
Instrument Name:Agilent CE
Column Name:COSMO(+), i.d.50 μm(anion)
Column Temperature:none
Flow Gradient:none
Flow Rate:none
Solvent A:none
Solvent B:none
Chromatography Type:CE