Summary of Study ST003026
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001880. The data can be accessed directly via it's Project DOI: 10.21228/M8Q72K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003026 |
Study Title | Untargeted Metabolomics Reveals Unique Biomolecular Signatures in Overweight and Obesity Using UHPLC-ESI-QTOF-MS Analysis |
Study Type | LC/MS/MS |
Study Summary | Aims: Obesity poses a multifaceted challenge to global public health, impacting individuals and society in various ways. Apart from the heightened susceptibility to chronic conditions such as diabetes, cardiovascular diseases, obesity significantly escalates healthcare costs. Effective public health strategies are essential for addressing issues related to early detection, diagnosis, and personalized treatment plans. This emphasizes the crucial need for a deep understanding of biochemical pathways, patient monitoring, and prognosis. In this context, metabolomics has become a valuable approach, focusing on the identification of metabolites in biofluids and tissues. Main Methods: In this study, an untargeted metabolomics-based method was employed to investigate metabolomic changes and their relationship to pathways in overweight and obese individuals. Plasma samples were collected from 29 healthy individuals with normal weight, 17 overweight individuals, and 28 obese individuals who met the inclusion criteria for the study. The plasma samples were analyzed using highly sensitive ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry. Results: Pantothenic acid and L-proline showed increased levels in the overweight group, whereas phenylacetaldehyde and glycerophosphocholine were notably decreased compared to the normal weight group. Conversely, the obese group exhibited elevated levels of specific metabolites, including L-leucine, L-tryptophan, phenylalanine, and tyrosine. On the contrary, the obese group demonstrated decreased levels of other metabolites such as 2,3-Diaminopropionic acid, and phenylacetaldehyde. Additionally, significant changes in metabolic pathways, such as pantothenate and CoA biosynthesis, and beta-alanine metabolism, were observed in the overweight group. In contrast, the obese group displayed significant alterations in phenylalanine and tyrosine metabolism, tryptophan metabolism, and beta oxidation of very long-chain fatty acids. Conclusion: The present investigation sheds light on the potential diagnostic significance of certain metabolites in obesity and the impact of their level changes on specific metabolic pathways. Additional studies are necessary to confirm the association of these metabolites in obesity and to confirm their diagnostic value. |
Institute | Sharjah Institute for Medical Research |
Department | Research institute of medical and health science |
Laboratory | Biomarker Discovery Group |
Last Name | Facility |
First Name | Core |
Address | M32, SIMR, College of Pharmacy, Health Sciences, University of Sharjah, Sharjah, UAE, Sharjah, 000, United Arab Emirates |
tims-tof@sharjah.ac.ae | |
Phone | +971 6 5057656 |
Submit Date | 2023-12-25 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2024-05-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004961 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Bruker Elute |
Column | Hamilton® Intensity Solo 2 C18 column (2.1 × 100 mm, 1.8 µm) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Bruker timsTOF |
Ion Mode | POSITIVE |
Units | AU |
Chromatography:
Chromatography ID: | CH003744 |
Chromatography Summary: | The LC-MS/MS analysis utilized an advanced ultra-high-performance liquid chromatography system (UHPLC) provided by Bruker Daltonik GmbH in Bremen, Germany. The analysis employed mobile phases A (water with 0.1% formic acid) and B (acetonitrile with 0.1% formic acid). The gradient program was: 0–2 min, 99% A: 1% B; 2–17 min, 99–1% A: 1–99% B; 17–20 min, 99% B: 1% A. The flow rate was fixed at 0.25 ml/min. Subsequently, 20–20.1 min 99% B to 99% A; 20.1–28.5 min, 99% A: 1% B at 0.35 ml/min flow rate; 28.5–30 min; 99% A: 1% B at 0.25 ml/min. The flow rate was maintained at a constant value throughout the analysis. The sample, in the form of a 10 μl aliquot, was injected into a Hamilton® Intensity Solo 2 C18 column (2.1 mm × 100 mm, 1.8 μm) for separation. The column oven temperature was set to 35 °C |
Instrument Name: | Bruker Elute |
Column Name: | Hamilton® Intensity Solo 2 C18 column (2.1 × 100 mm, 1.8 µm) |
Column Temperature: | 35 ◦C |
Flow Gradient: | The gradient program was: 0–2 min, 99% A: 1% B; 2–17 min, 99–1% A: 1–99% B; 17–20 min, 99% B: 1% A. The flow rate was fixed at 0.25 ml/min. Subsequently, 20–20.1 min 99% B to 99% A; 20.1–28.5 min, 99% A: 1% B at 0.35 ml/min flow rate; 28.5–30 min; 99% A: 1% B at 0.25 ml/min. |
Flow Rate: | The flow rate was maintained at a constant value throughout the analysis |
Solvent A: | Water (0.1% Formic Acid) |
Solvent B: | ACN (0.1% Formic Acid) |
Chromatography Type: | Reversed phase |