Summary of Study ST003101
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001924. The data can be accessed directly via it's Project DOI: 10.21228/M81B11 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003101 |
Study Title | Parallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens |
Study Summary | We used a novel pheromone detection method to quantify retinue pheromone (QRP) concurrently with shotgun metabolomics and lipidomics analysis to determine what changes in pheromones and small molecules may underpin differences in age, laying status, and acceptance by workers in honey bee queens. |
Institute | Life Sciences Institute, The University of British Columbia |
Last Name | Alcazar Magana |
First Name | Armando |
Address | 2350 Health Sciences Mall, Vancouver, BC, V6T1Z3, Canada |
armando.alcazarmagana@ubc.ca | |
Phone | 5416097172 |
Submit Date | 2024-02-20 |
Num Groups | 4 |
Total Subjects | 40 |
Num Females | 40 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2024-05-24 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN005072 | AN005073 | AN005074 | AN005075 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
Column | Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) | Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | QTOF | QTOF | QTOF | QTOF |
MS instrument name | Bruker Impact II | Bruker Impact II | Bruker Impact II | Bruker Impact II |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Rel Abundance | Rel Abundance | Rel Abundance | Rel Abundance |
Chromatography:
Chromatography ID: | CH003831 |
Chromatography Summary: | Separation of compounds was achieved using a multigradient method on an Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) (GL Sciences) equipped with a Ph-3 guard column (2 µm, 2.1 x 10 mm |
Instrument Name: | Thermo Vanquish |
Column Name: | Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) |
Column Temperature: | 55 |
Flow Gradient: | 0 min (5% B), 0–1 min (5% B), 1–8 min (35% B), 8–10.5 min (99% B), 10.5–14 min (99% B), 14–14.5 min (5% B), and 14.5–18 min (5% B) |
Flow Rate: | 0.3 ml/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% methanol; 0.1% formic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH003832 |
Chromatography Summary: | Lipids were separated on an ACQUITY UPLC CSH C18 analytical column (130Å, 1.7 µm, 2.1 mm X 100 mm, Waters) with a multi-step elution gradient optimized to resolve polar lipids such as hydroxylated fatty acids analyzed. |
Instrument Name: | Thermo Vanquish |
Column Name: | Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) |
Column Temperature: | 65 |
Flow Gradient: | 0 min (20% B), 0–2 min (20% B), 2–11 min (80% B), 11–11.5 min (99% B), 11.5–13.2 min (99% B), 13.2–14 min (5% B), and 14–18 min (5% B). |
Flow Rate: | 0.4 ml/min |
Solvent A: | 100% water; 10 mM ammonium formate; 0.1% formic acid |
Solvent B: | 10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid |
Chromatography Type: | Reversed phase |