Summary of Study ST003101

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001924. The data can be accessed directly via it's Project DOI: 10.21228/M81B11 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003101
Study TitleParallel pheromonal, metabolite, and lipid analyses reveal patterns associated with early life transitions and ovary activation in honey bee (Apis mellifera) queens
Study SummaryWe used a novel pheromone detection method to quantify retinue pheromone (QRP) concurrently with shotgun metabolomics and lipidomics analysis to determine what changes in pheromones and small molecules may underpin differences in age, laying status, and acceptance by workers in honey bee queens.
Institute
Life Sciences Institute, The University of British Columbia
Last NameAlcazar Magana
First NameArmando
Address2350 Health Sciences Mall, Vancouver, BC, V6T1Z3, Canada
Emailarmando.alcazarmagana@ubc.ca
Phone5416097172
Submit Date2024-02-20
Num Groups4
Total Subjects40
Num Females40
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-05-24
Release Version1
Armando Alcazar Magana Armando Alcazar Magana
https://dx.doi.org/10.21228/M81B11
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005072 AN005073 AN005074 AN005075
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Vanquish Thermo Vanquish
Column Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Bruker Impact II Bruker Impact II Bruker Impact II Bruker Impact II
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Rel Abundance Rel Abundance Rel Abundance Rel Abundance

Chromatography:

Chromatography ID:CH003831
Chromatography Summary:Separation of compounds was achieved using a multigradient method on an Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm) (GL Sciences) equipped with a Ph-3 guard column (2 µm, 2.1 x 10 mm
Instrument Name:Thermo Vanquish
Column Name:Inertsil Ph-3 UHPLC column (2 µm, 150 x 2.1 mm)
Column Temperature:55
Flow Gradient:0 min (5% B), 0–1 min (5% B), 1–8 min (35% B), 8–10.5 min (99% B), 10.5–14 min (99% B), 14–14.5 min (5% B), and 14.5–18 min (5% B)
Flow Rate:0.3 ml/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% methanol; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH003832
Chromatography Summary:Lipids were separated on an ACQUITY UPLC CSH C18 analytical column (130Å, 1.7 µm, 2.1 mm X 100 mm, Waters) with a multi-step elution gradient optimized to resolve polar lipids such as hydroxylated fatty acids analyzed.
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC CSH C18 (100 x 2.1mm,1.7um)
Column Temperature:65
Flow Gradient:0 min (20% B), 0–2 min (20% B), 2–11 min (80% B), 11–11.5 min (99% B), 11.5–13.2 min (99% B), 13.2–14 min (5% B), and 14–18 min (5% B).
Flow Rate:0.4 ml/min
Solvent A:100% water; 10 mM ammonium formate; 0.1% formic acid
Solvent B:10% acetonitrile/90% isopropanol; 10 mM ammonium formate; 0.1% formic acid
Chromatography Type:Reversed phase
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