Summary of Study ST003297
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002048. The data can be accessed directly via it's Project DOI: 10.21228/M8WG0S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003297 |
| Study Title | Metabolomic profiling of cultured TRAMP-C2 cells in the presence or absence of PD-L1. |
| Study Summary | Recent evidence suggests that PD-L1, well-known as the ligand of the immune inhibitory receptor PD-1, can have cell-intrinsic effects in cancer and immune cells. One such cell-intrinsic effect is modulation of cellular metabolism, including regulation of mTOR activity and glycolysis. Here, we analyzed the metabolome of cultured mouse prostate cancer cells (TRAMP-C2) expressing PD-L1 or with PD-L1 deleted via CRISPR/Cas9. |
| Institute | University of Ottawa |
| Last Name | Hodgins |
| First Name | Jonathan |
| Address | 451 Smyth Rd, Ottawa, ON K1H 8M5, Canada |
| jonathanhodgins17@gmail.com | |
| Phone | 613-562-5800 |
| Submit Date | 2024-05-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005401 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity II |
| Column | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6470A |
| Ion Mode | NEGATIVE |
| Units | uM |
Chromatography:
| Chromatography ID: | CH004094 |
| Instrument Name: | Agilent 1290 Infinity II |
| Column Name: | Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) |
| Column Temperature: | 35℃ |
| Flow Gradient: | 0-2.5 min 100% A, 2.5-7.5 min 100%-80% A; 7.5-13 min 80%-55% A, 13-20 min 55%-1% A, 20-24 min holding at 1% A, 24.05-31.5 min backflushing the column with 90% acetonitrile using channel C and then 32.25-40 min equilibrating the column with 100% A |
| Flow Rate: | 0.25 mL/min |
| Solvent A: | 100% Water; 10 mM tributylamine; 5 uM Agilent InfinityLab deactivator |
| Solvent B: | 100% Methanol; 10 mM tributylamine; 5 uM Agilent InfinityLab deactivator |
| Chromatography Type: | Reversed phase |
