Summary of Study ST003361
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002087. The data can be accessed directly via it's Project DOI: 10.21228/M8V825 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003361 |
| Study Title | Bile acid–induced gut mitochondrial dysfunction promotes Enterobacteriaceae colonization and associates with dysbiosis in Crohn's disease |
| Study Summary | Here, we investigated whether bile acids influence gut colonization by Enterobacteriaceae. In an external human dataset, we found association between mucosal Enterobacteriaceae and the expression of mitochondrial damage–related genes. In a murine model, increased intestinal BA availability enhanced colonization by E. coli in an oxygen-dependent manner. Overall, this study establishes BAs as a potential bridge that facilitates interactions between commensals and the host epithelium, laying the essential foundation for future investigations into various intestinal diseases. |
| Institute | University of British Columbia |
| Last Name | Finlay |
| First Name | Brett |
| Address | office: MSL 333 lab: MSL 363 |
| bfinlay@msl.ubc.ca | |
| Phone | 604-822-2210 |
| Submit Date | 2024-07-30 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-08-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005503 |
|---|---|
| Chromatography ID | CH004185 |
| MS ID | MS005229 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Vanquish |
| Column | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker Impact II |
| Ion Mode | NEGATIVE |
| Units | Relative abundance |
Chromatography:
| Chromatography ID: | CH004185 |
| Chromatography Summary: | A longer ACQUITY UPLC CSH C18 analytical column (130Å, 1.7 µm, 2.1 mm X 150 mm, Waters) was used to achieve chromatographic separation, with a multistep elution gradient. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Gradient: | 0 min 30% B, 2.5 min 30% B, 10 min 55% B, 11 min 99%B, 13min 99% B, 13.5 min 30% B, 17 min 30% B |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic |
| Chromatography Type: | Reversed phase |
