Summary of Study ST003479

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002137. The data can be accessed directly via it's Project DOI: 10.21228/M8CZ33 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST003479
Study TitleFatty acid analysis of similarly sized MC38 tumors from control-diet- or high-fat-diet-fed C57BL/6 mice revealed an increase in tumoral oleic acid in high-fat-diet-fed mice.
Study SummarySimilarly sized MC38 tumors from control-diet- and high-fat-diet-fed mice were subject to fatty acid analysis via GC-FID. These data revealed an increase in oleic acid in tumors from high-fat-diet-fed mice. Oleic acid was the only fatty acid to exhibit this trend.
Institute
Stanford University
Last NameBagchi
First NameSreya
Address3373 Hillview Avenue
Emailbagchipuja@gmail.com
Phone4086214773
Submit Date2024-08-12
Analysis Type DetailOther
Release Date2024-10-18
Release Version1
Sreya Bagchi Sreya Bagchi
https://dx.doi.org/10.21228/M8CZ33
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Chromatography:

Chromatography ID:CH004335
Chromatography Summary:GC-FID was performed on an Agilent 6890N GC coupled to a FID ( flame ionization detector). The FID detector was operated at 260 °C. The hydrogen flow to the detector was 30 mL/min and air flow was 400 mL/min. The sampling rate of the FID was 20 Hz.
Methods Filename:MC38_fatty_acid_GC_protocol.pdf
Instrument Name:Agilent 6890N
Column Name:Agilent DB-23 (60m x 0.25mm, 0.25um)
Column Temperature:250
Flow Gradient:Temperature-programmed GC
Flow Rate:1.5 mL/min
Internal Standard:pentadecanoic acid (15:0)
Solvent A:3 M methanolic hydrochloric acid
Solvent B:100% hexane
Chromatography Type:GC
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