Summary of Study ST003492

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002141. The data can be accessed directly via it's Project DOI: 10.21228/M8VZ4T This work is supported by NIH grant, U2C- DK119886.

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Study IDST003492
Study TitleMetabolomic profiling of the secretome in normal primary human pulmonary artery smooth muscle cells
Study SummaryGrowth medium of human pulmonary artery smooth muscle cells (PASMCs) was conditioned for 24 hours and analyzed by LC-MS, termed conditioned medium (CM). Comparing to fresh complete vascular smooth muscle cell growth medium (GM), LC-MS profiling screened 138 metabolites and identified that three branched chain alpha-ketoacids, namely KIC, KIV, and KMV are the most abundant metabolites in CM of human PASMCs.
Institute
Peking University
DepartmentDepartment of Toxicology
Last NameXiao
First NameWusheng
Address38 Xueyuan Rd, Haidian District, Beijing, Beijing, 100191, China
Emailwxiao@bjmu.edu.cn
Phone+1-010-82801628
Submit Date2023-08-07
Analysis Type DetailMALDI
Release Date2024-10-04
Release Version1
Wusheng Xiao Wusheng Xiao
https://dx.doi.org/10.21228/M8VZ4T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005732
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Merck ZIC-pHILIC(150 mm × 2.1 mm,3.5um)
MS Type MALDI
MS instrument type Triple quadrupole
MS instrument name Thermo Q Exactive Focus
Ion Mode UNSPECIFIED
Units ion intensity

Chromatography:

Chromatography ID:CH004351
Chromatography Summary:Vanquish ultra-high performance liquid chromatography system coupled to a Q Exactive mass spectrometer
Instrument Name:Thermo Vanquish
Column Name:Merck ZIC-pHILIC(150 mm × 2.1 mm,3.5um)
Column Temperature:4
Flow Gradient:gradient (%B) was 0 min, 80%; 20 min, 20%; 20.5 min, 80%; 28 min, 80%; and 42 min
Flow Rate:100
Solvent A:100% water; 20 mM ammonium carbonate; 0.1% ammonium hydroxide
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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