Summary of Study ST003492
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002141. The data can be accessed directly via it's Project DOI: 10.21228/M8VZ4T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST003492 |
Study Title | Metabolomic profiling of the secretome in normal primary human pulmonary artery smooth muscle cells |
Study Summary | Growth medium of human pulmonary artery smooth muscle cells (PASMCs) was conditioned for 24 hours and analyzed by LC-MS, termed conditioned medium (CM). Comparing to fresh complete vascular smooth muscle cell growth medium (GM), LC-MS profiling screened 138 metabolites and identified that three branched chain alpha-ketoacids, namely KIC, KIV, and KMV are the most abundant metabolites in CM of human PASMCs. |
Institute | Peking University |
Department | Department of Toxicology |
Last Name | Xiao |
First Name | Wusheng |
Address | 38 Xueyuan Rd, Haidian District, Beijing, Beijing, 100191, China |
wxiao@bjmu.edu.cn | |
Phone | +1-010-82801628 |
Submit Date | 2023-08-07 |
Analysis Type Detail | MALDI |
Release Date | 2024-10-04 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN005732 |
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Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Thermo Vanquish |
Column | Merck ZIC-pHILIC(150 mm × 2.1 mm,3.5um) |
MS Type | MALDI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Q Exactive Focus |
Ion Mode | UNSPECIFIED |
Units | ion intensity |
Chromatography:
Chromatography ID: | CH004351 |
Chromatography Summary: | Vanquish ultra-high performance liquid chromatography system coupled to a Q Exactive mass spectrometer |
Instrument Name: | Thermo Vanquish |
Column Name: | Merck ZIC-pHILIC(150 mm × 2.1 mm,3.5um) |
Column Temperature: | 4 |
Flow Gradient: | gradient (%B) was 0 min, 80%; 20 min, 20%; 20.5 min, 80%; 28 min, 80%; and 42 min |
Flow Rate: | 100 |
Solvent A: | 100% water; 20 mM ammonium carbonate; 0.1% ammonium hydroxide |
Solvent B: | 100% acetonitrile |
Chromatography Type: | HILIC |