Summary of Study ST003531
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002173. The data can be accessed directly via it's Project DOI: 10.21228/M8R23J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003531 |
| Study Title | Micropeptide hSPAR, a glutamine regulator, suppresses tumor growth via TRIM21-P27KIP1-mTOR pathway - human MDA-MB-231 breast cancer cell |
| Study Summary | hSPAR, a microprotein, is capable of specifically inhibiting the mTOR signaling activity and cell proliferation in MDA-MB-231 cells. Glutamine, an essential amino acid, plays a crucial role in regulating the mTOR signal. Our data indicate that in MDA-MB-231 cells, overexpressing hSPAR inhibits glutamine uptake, consequently suppressing the activation of the mTOR signaling. |
| Institute | University of Science and Technology of China |
| Last Name | Wang |
| First Name | Wei |
| Address | Division of Life Sciences and Medicine, 443 Huangshan Road, Hefei city, Anhui Province |
| WW571@mail.ustc.edu.cn | |
| Phone | 18604523231 |
| Submit Date | 2024-10-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005800 | AN005801 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | SCIEX ExionLC AD | SCIEX ExionLC AD |
| Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | QTRAP | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | ng/mg of cells | ng/mg of cells |
Chromatography:
| Chromatography ID: | CH004405 |
| Methods Filename: | USTC_Protocol.pdf |
| Instrument Name: | SCIEX ExionLC AD |
| Column Name: | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Column Temperature: | 40°C |
| Flow Gradient: | The gradient was started at 90% B at 0-1.2 min, decreased to 60% B at 9 min, 40% B at 10-11 min, finaly ramped back to 90% B at 11.01-15 min |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 2 mM ammonium acetate; 0.04% formic acid |
| Solvent B: | 100% acetonitrile; 2 mM ammonium acetate; 0.04% formic acid |
| Chromatography Type: | HILIC |
