Summary of Study ST003808
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002382. The data can be accessed directly via it's Project DOI: 10.21228/M8QZ7Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003808 |
| Study Title | Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division |
| Study Summary | Peroxisomes play a crucial role in cellular metabolism. Glucose-6-phosphate dehydrogenase (G6PD), the gatekeeper enzyme of the pentose phosphate pathway, is primarily localized in the cytosol. However, studies have reported its presence in peroxisomes as well. This project aims to determine the function of G6PD on the peroxisomal membrane. In this study, we overexpressed G6PD in either the cytosol or on the peroxisomal membrane of mammary epithelial stem-like cells (hMECs). By comparing their lipidomic profiles, we found that peroxisomal membrane-associated G6PD provides NADPH, which feeds into peroxisomal ether lipid synthesis. |
| Institute | University of Helsinki |
| Department | Faculty of Biological and Environmental Sciences |
| Laboratory | Katajisto Laboratory |
| Last Name | Hien |
| First Name | Bui |
| Address | Biocenter 1, Viikinkaari 9 |
| hien.bui@helsinki.fi | |
| Phone | +358294159407 |
| Submit Date | 2025-03-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, mzdata.xml |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2025-03-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006261 | AN006262 |
|---|---|---|
| Chromatography ID | CH004749 | CH004750 |
| MS ID | MS005963 | MS005964 |
| Analysis type | MS | MS |
| Chromatography type | GC | Reversed phase |
| Chromatography system | Shimadzu GC-2010 | Agilent 1290 Infinity |
| Column | Phenomenex Zebron ZB-wax capillary (30m x 0.25mm, 0.25um) | Phenomenex Luna Omega C18 (50 x 2.1mm, 1.6um, 100 Å) |
| MS Type | EI | ESI |
| MS instrument type | GC-FID & GC-MSD | Triple quadrupole |
| MS instrument name | Shimadzu QP2010 Plus | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pmol/sample | peak area |
Chromatography:
| Chromatography ID: | CH004749 |
| Chromatography Summary: | The samples were injected into a GC-2010 Plus gas chromatograph (Shimadzu Scientific Instruments, Kyoto, Japan) with flame-ionization detector (FID) for quantification of the analytes. Both GC systems were equipped with Zebron ZB-wax capillary columns (30 m, 0.25 mm ID and film thickness 0.25 μm; Phenomenex, Torrence CA, USA). GC-FID chromatographic peak areas (in the TXT files; analyte retention times in Table 1) were converted to mol% using the theoretical correction factors for FID (6), and the FOHs were calculated as µmol/10^6 cells. |
| Instrument Name: | Shimadzu GC-2010 |
| Column Name: | Phenomenex Zebron ZB-wax capillary (30m x 0.25mm, 0.25um) |
| Column Temperature: | 280 °C for FID and 210 °C for MSD. |
| Flow Gradient: | NA |
| Flow Rate: | 1.8 ml min–1 for FID and 1.0 ml min–1 for MSD |
| Solvent A: | NA |
| Solvent B: | NA |
| Chromatography Type: | GC |
| Chromatography ID: | CH004750 |
| Chromatography Summary: | The method used acetonitrile/water/isopropanol-based solvent system, Agilent 1290 Infinity HPLC (Agilent Technologies, Santa Clara, CA) equipped with a Luna Omega C18 100 Å (50 x 2.1 mm, 1.6 μm) column (Phenomenex) and Agilent 6490 Triple Quad LC/MS with iFunnel Technology. The lipids species were identified and quantified using lipid class-specific precursor ion and neutral loss detection modes. PE plasmalogens (PE P) were identified with alkenyl chain (16:0, 18:0, 18:1)-specific scans and quantified from MS- scan. - Due to a contamination during the sample run, some samples were removed from analysis |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Phenomenex Luna Omega C18 (50 x 2.1mm, 1.6um, 100 Å) |
| Column Temperature: | 25 |
| Flow Gradient: | 32% B at 0.0 min, 32% B at 1.5 min, 45% B at 4.0 min, 52% B at 5.0 min, 58% B at 8.0 min, 66% B at 11.0 min, 70% B at 14.0 min, 75% B at 18.0 min, 97% B at 21.0 min, 97% B at 25.0 min, 32% B at 26.0 min, 32% B at 32.0 min. flow gradient field is a linear gradient |
| Flow Rate: | 0.200 mL/min |
| Solvent A: | 40% water/60% acetonitrile; 0.1% formic acid; 10mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium formate |
| Chromatography Type: | Reversed phase |
