Summary of Study ST004042
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002534. The data can be accessed directly via it's Project DOI: 10.21228/M83G11 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST004042 |
| Study Title | Untargeted Metabolomics Identifies Oncometabolites Induced by P2-HNF4α Overexpression in Hepatocytes |
| Study Summary | This study explores the metabolic impact of P2-HNF4α overexpression in primary mouse hepatocytes. Using untargeted metabolomics, we compared metabolite profiles between control and P2-HNF4α-overexpressing cells to identify metabolic pathways potentially altered in liver disease and HCC. |
| Institute | University of Tennessee Health Science Center |
| Department | IMM |
| Laboratory | Mahan Lab |
| Last Name | Fekry |
| First Name | Baharan |
| Address | 1825 Pressler St, Houston TX 77030. USA. |
| Baharan.Fekry@uth.tmc.edu | |
| Phone | 18434693199 |
| Submit Date | 2025-07-03 |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-08-05 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006681 |
|---|---|
| Chromatography ID | CH005077 |
| MS ID | MS006380 |
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1200 Series HPLC coupled to Agilent 6538 UHD Accurate-Mass Q-TOF |
| Column | Agilent ZORBAX Extend-C18 Rapid Resolution HT (2.1 × 50 mm, 1.8 µm) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6538 QTOF |
| Ion Mode | POSITIVE |
| Units | LC/MS Peak intensity |
Chromatography:
| Chromatography ID: | CH005077 |
| Chromatography Summary: | Liquid chromatography was performed using an Agilent 1200 Series HPLC system equipped with an Agilent ZORBAX Extend-C18 Rapid Resolution HT column (2.1 × 50 mm, 1.8 µm). The mobile phases consisted of Buffer A (2% acetonitrile, 98% water, 0.1% formic acid) and Buffer B (80% acetonitrile, 20% water, 0.1% formic acid). A linear gradient from 25% to 90% Buffer B was applied over 20 minutes at a flow rate of 0.2 mL/min to achieve metabolite separation prior to MS analysis. |
| Instrument Name: | Agilent 1200 Series HPLC coupled to Agilent 6538 UHD Accurate-Mass Q-TOF |
| Column Name: | Agilent ZORBAX Extend-C18 Rapid Resolution HT (2.1 × 50 mm, 1.8 µm) |
| Column Temperature: | 21°C |
| Flow Gradient: | 25% to 90% Solvent B over 20 minutes |
| Flow Rate: | 0.2 mL/min |
| Solvent A: | 2% Acetonitrile/98% Water; 0.1% formic acid |
| Solvent B: | 80% Acetonitrile/20% Water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
