Summary of Study ST004204
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002650. The data can be accessed directly via it's Project DOI: 10.21228/M83V73 This work is supported by NIH grant, U2C- DK119886. See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST004204 |
| Study Title | The growth and virulence of S. aureus is inhibited by C. striatum by inhibiting its arginine biosynthesis pathway |
| Study Summary | Background: Patients with chronic rhinosinusitis (CRS) exhibit signs of nasal microbiota dysbiosis, specifically manifested by an increase in the relative abundance of Staphylococcus species and a decrease in the relative abundance of Corynebacterium species. This alteration may be closely associated with the pathogenesis of CRS. Understanding how commensal bacteria interact with pathogenic bacteria can help us reconstruct the homeostasis of nasal microbiota and promote nasal health. Method and Results: We conducted an in vitro co-culture model of Staphylococcus aureus and Corynebacterium striatum. Through metabolomic analysis, we discovered that the metabolic pathways of S. aureus were downregulated considerably during co-culture, especially for the arginine biosynthesis pathway. We later conducted validation experiments involving arginine supplementation and air-liquid interface culture of human nasal epithelial cells. Conclusion: The growth and virulence of S. aureus is inhibited by C. striatum by inhibiting its arginine biosynthesis pathway. |
| Institute | Sun Yat-sen University |
| Department | Otorhinolaryngology, First Affiliated Hospital of Sun Yat-sen University |
| Laboratory | Otorhinolaryngology Institute of Sun Yat-sen University |
| Last Name | Xinyu |
| First Name | Zhang |
| Address | No.74 Zhongshan Er Road |
| zhangxy2236@mail.sysu.edu.cn | |
| Phone | +86 15920583447 |
| Submit Date | 2025-09-04 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006990 | AN006991 |
|---|---|---|
| Chromatography ID | CH005307 | CH005307 |
| MS ID | MS006687 | MS006688 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Hypersil Gold aQ C18 (100x2.1mm, 1.9um) | Thermo Hypersil Gold aQ C18 (100x2.1mm, 1.9um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | relative abundance | relative abundance |
Chromatography:
| Chromatography ID: | CH005307 |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Hypersil Gold aQ C18 (100x2.1mm, 1.9um) |
| Column Temperature: | 40℃ |
| Flow Gradient: | 0 to 1.5 min: remain 98% A(A: 0.1% formic acid) and 2% B (B: methyl alcohol) solution; 1.5 to 3 min: 98%A to 15%A, 2%B to 85%B ; 3 to 10 min: 15%A to 0%A, 85%B to 100% B; 10.1 to 12 min: remain 98%A and 2% B |
| Flow Rate: | 0.2mL/min |
| Solvent A: | 100% water; 0.1% Formic acid |
| Solvent B: | 100% Methyl alcohol |
| Chromatography Type: | Reversed phase |
