Summary of Study ST000055
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000052. The data can be accessed directly via it's Project DOI: 10.21228/M8H01X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000055 |
| Study Title | Effect of kinase inhibitors on FLT3-ITD AML cell metabolomes |
| Study Summary | FLT3-ITD AML cells (MR2) obtained from mice were treated with two MEK kinase inhibitors (GSK and AZD) at 10 uM versus media only control. Conditioned media aliquots and cellular fractions comprised of two aliquots (tecnical replicates) for LC-MS metabolomic and one for Western blot analyses were collected at 0, 4, 24, and 48 hours. The experiment was repeated three times. HPLC-MS data were acquired for 24 samples from one biological experiment. |
| Institute | University of North Carolina |
| Department | Systems and Translational Sciences |
| Laboratory | Sumner Lab |
| Last Name | Sumner |
| First Name | Susan |
| Address | Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081 |
| susan_sumner @unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2014-06-06 |
| Num Groups | 12 |
| Total Subjects | 24 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Uploaded File Size | 1.2 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-06-01 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO000057 |
| Collection Summary: | Conditioned Medias:Cellular Fraction_LC-MS:Cellular Fraction_WB |
| Collection Protocol Comments: | Processed simultaneously/time point:Processed simultaneously/time point:Processed simultaneously/time point |
| Sample Type: | conditioned treatment media |
| Collection Method: | Total contents pipette-transferred to tubes & centrifuged at 1800 rpm for 5 minutes. Medias transferred into aliquot tubes.:Cellular fractions pelleted & resuspended in 1X PBS. Aliqotted into collection vials based on cell counts. Re-centrifuged at 8,000 rpm for 3 min at 4 °C. PBS was aspirated off pellets for storage.:Cellular fractions pelleted & resuspended in 1X PBS. Aliqotted into collection vial based on cell counts. Re-centrifuged at 8,000 rpm for 3 min at 4 °C. PBS was aspirated off pellets for storage. |
| Collection Location: | RTI RCMRC Tissue culture suite:RTI RCMRC Wet lab:RTI RCMRC Wet lab |
| Collection Frequency: | 0h, 4h, 24h and 48h:0h, 4h, 24h and 48h:0h, 4h, 24h and 48h |
| Collection Duration: | 10 minutes:10 minutes:5 minutes |
| Collection Time: | 0h, 4h, 24h and 48h:0h, 4h, 24h and 48h:0h, 4h, 24h and 48h |
| Volumeoramount Collected: | 1 mL x 5 aliquots:2x10^7 cells:2x10^6 cells |
| Storage Conditions: | -80C:-80C:-80C |
| Collection Vials: | 15 mL conical tubes:15 mL conical tubes:15 mL conical tubes |
| Storage Vials: | 1.5 mL Eppendorf Protein Lo-Bind tubes:1.5 mL Eppendorf Protein Lo-Bind tubes:1.5 mL Eppendorf Protein Lo-Bind tubes |
| Collection Tube Temp: | chilled in ice:chilled in ice:chilled in ice |
