Summary of study ST000116

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000105. The data can be accessed directly via it's Project DOI: 10.21228/M8W300 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000116
Study TitleComparative metabolomics analysis of the key metabolic nodes in propionic acid synthesis in Propionibacterium acidipropionici
Study Typestrains comparison
Study SummaryThe parental P. acidipropionici and its genome-shuffled mutant were compared via metabolomics to find the key metabolic nodes influencing PA production.
Institute
Jiangnan University
DepartmentKey Laboratory of Carbohydrate Chemistry and Biotechnology
Last NameGuan
First NameNingzi
Address1800 Lihu Ave, Binhu, Wuxi, Jiangsu, China
Emailcaimo-zi@163.com
Submit Date2014-11-14
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2015-02-03
Release Version1
Ningzi Guan Ningzi Guan
https://dx.doi.org/10.21228/M8W300
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO000119
Collection Summary:The cells were quenched by immediately adding a threefold volume of pre-chilled 60% (v/v) methanol solution containing 70 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid, and pelleted in a centrifuge (4,000 × g, ?4°C, 10 min)
Collection Protocol Filename:Protocol_Methods-G.docx
Sample Type:cell
Collection Time:Samples were collected at the middle exponential phase, late exponential phase, and the end of fermentation
Storage Conditions:-80°C
Collection Vials:50mL centrifuge tube
Storage Vials:50mL centrifuge tube
Collection Tube Temp:pre-chilled at -80°C
Additives:pre-chilled 60% (v/v) methanol solution containing 70 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid
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