Summary of Study ST000284
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000226. The data can be accessed directly via it's Project DOI: 10.21228/M8FG61 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000284 |
| Study Title | Colorectal Cancer Detection Using Targeted Serum Metabolic Profiling |
| Study Summary | Colorectal cancer (CRC) is one of the most prevalent and deadly cancers in the world. Despite an expanding knowledge of its molecular pathogenesis during the past two decades, robust biomarkers to enable screening, surveillance, and therapy monitoring of CRC are still lacking. In this study, we present a targeted liquid chromatography-tandem mass spectrometry-based metabolic profiling approach for identifying biomarker candidates that could enable highly sensitive and specific CRC detection using human serum samples. In this targeted approach, 158 metabolites from 25 metabolic pathways of potential significance were monitored in 234 serum samples from three groups of patients (66 CRC patients, 76 polyp patients, and 92 healthy controls). Partial least squares-discriminant analysis (PLS-DA) models were established, which proved to be powerful for distinguishing CRC patients from both healthy controls and polyp patients. Receiver operating characteristic curves generated based on these PLS-DA models showed high sensitivities (0.96 and 0.89, respectively, for differentiating CRC patients from healthy controls or polyp patients); good specificities (0.80 and 0.88), and excellent areas under the curve (0.93 and 0.95) were also obtained. Monte Carlo cross validation (MCCV) was also applied, demonstrating the robust diagnostic power of this metabolic profiling approach. |
| Institute | University of Washington |
| Department | Anesthesiology and Pain Medicine |
| Laboratory | Northwest Metabolomics Research Center |
| Last Name | Gu |
| First Name | Haiwei |
| Address | 850 Republican St. |
| haiwei@uw.edu | |
| Phone | 7654919481 |
| Submit Date | 2015-12-15 |
| Num Groups | 3 |
| Total Subjects | 234 |
| Num Males | 118 |
| Num Females | 116 |
| Publications | Colorectal Cancer Detection Using Targeted Serum Metabolic Profiling, J. Proteome. Res., 2014, 13, 4120-4130 |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-12-16 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO000298 |
| Collection Summary: | Patient recruitment and sample collection protocols were University and Indiana University Boards. Informed according (both were evaluated, and blood samples from the overnight fasting and bowel polyp status were polyp patients (n = 76), and healthy controls (n = 92) biopsied tissue. Patients were age- and the gender/age group suggesting sample was allowed to clot for 45 min and then All samples were stored at -80C |
| Sample Type: | Blood serum |
| Blood Serum Or Plasma: | Serum |
