Summary of Study ST000353

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000282. The data can be accessed directly via it's Project DOI: 10.21228/M8G308 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000353
Study TitleThe Development of Metabolomic Markers in African Bermudagrass (C. transvaalensis) for Sting Nematode (Belonolaimus longicaudatus) Response
Study TypeDisease response in terms of nematode reproduction and root weight
Study SummaryThe objective of the proposed pilot study is to identify metabolites up- and down-regulated in African bermudagrass that are tolerant and sensitive to the sting nematode and develop metabolomic markers for the highest expressed metabolites associated with tolerance. Future work will include additional accessions and species of bermudagrass, and testing under field conditions. Bermudagrass accessions identified as tolerant or sensitive by Pang et al. (2011) will be assessed under controlled greenhouse conditions to identify metabolites linked to sting nematode tolerance. Nematode response will be quantified through determination of root length and weight and the number of nematodes present 136 days after inoculation. Higher root length and weight indicate tolerance or resistance. Higher nematode counts indicate greater reproduction (i.e. a susceptible plant), while lower counts indicate that the accession may have some resistance. Metabolites from root tissue of these accessions will be compared to identify those associated with tolerance/resistance, and those that are associated with nematode infestation by comparing inoculated plants to uninoculated controls. Metabolomic markers will then be developed for the metabolites associated with tolerance/resistance. These markers will be used to guide future screening of bermudagrass accessions for breeding nematode-tolerant or -resistant varieties.
Institute
University of Florida
DepartmentSECIM
LaboratoryTurfgrass Breeding
Last NameBenda
First NameNicole
Address2005 SW 23rd St
Emailnbenda@ufl.edu
Phone352-792-4561
Submit Date2015-11-13
Num Groups3
Total Subjects42
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2017-07-10
Release Version1
Nicole Benda Nicole Benda
https://dx.doi.org/10.21228/M8G308
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO000367
Collection Summary:Rinsed roots were cut off the plant, placed into paper envelopes, and immediately placed into liquid nitrogen. Roots were stored at -80°C until freeze-drying. Freeze-dried roots were held at room temperature in ziploc bags with silica gel packets. They were then frozen in liquid nitrogen and ground to a powder in a tissue homogenizer with ball bearings (Geno/Grinder 2010), and then stored in 2 ml snap-cap plastic tubes at room temperature.
Sample Type:plant root tissue
Collection Method:whole plant destruction
Collection Location:greenhouse
Collection Frequency:once
Collection Duration:1-2 minutes to cut off and rinse the roots prior to placement into liquid nitrogen
Collection Time:136 days after inoculation
Volumeoramount Collected:0.2-0.5 grams of root tissue, dry weight
Storage Conditions:1)-80C until freeze-drying. 2) Held at room temperature in ziploc bags with silica packets. 3) Frozen in liquid nitrogen and ground using a Geno/Grinder 2010. 4) Transferred to 2 mL tubes and held at room temperature.
Collection Vials:Paper coin envelopes
Storage Vials:2 ml snap-cap plastic tubes
Collection Tube Temp:liquid nitrogen (-196°C)
Additives:none
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