Summary of Study ST000384

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000301. The data can be accessed directly via it's Project DOI: 10.21228/M84P4J This work is supported by NIH grant, U2C- DK119886.


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Study IDST000384
Study TitleMetabolomic profiles in P. gingivalis cells treated with pABA
Study SummaryMany human infections are polymicrobial in origin, and synergistic interactions among community inhabitants control colonization and pathogenic potential (Murray et al., 2014). However, few interspecies interactions have been functionally dissected at the molecular level or characterized on a systems level. Periodontitis, which is the sixth most prevalent infectious disease worldwide (Kassebaum et al., 2014), is associated with a dysbiotic microbial community, and the keystone pathogen Porphyromonas gingivalis forms synergistic communities with the accessory pathogen Streptococcus gordonii (Lamont and Hajishengallis, 2015). P. gingivalis and S. gordonii communicate through co-adhesion and metabolite perception, and close association between P. gingivalis and S. gordonii results in significant changes in the expressed proteomes of both organisms (Kuboniwa et al., 2012, Hendrickson et al., 2012). Here we show that streptococcal 4 aminobenzoate/para-amino benzoic acid (pABA) is required for maximal accumulation of P. gingivalis in communities with S. gordonii. Exogenous pABA upregulates production of fimbrial interspecies adhesins and of a tyrosine phosphorylation-dependent signaling system in P. gingivalis. Consequently, fimbrial-dependent attachment and invasion of epithelial cells by P. gingivalis is also increased by pABA. Moreover, trans-omics studies performed by proteomics and metabolomics showed that pABA induces metabolic shifts within P. gingivalis, predominantly folate derivative biosynthesis. In a murine oral infection model, pABA increased colonization and survival of P. gingivalis, but did not increase virulence. The results establish streptococcal pABA as a major component of the interspecies S. gordonii-P. gingivalis interaction which regulates distinct aspects of polymicrobial synergy.
Osaka University
DepartmentGraduate School of Dentistry
Last NameKuboniwa
First NameMasae
Address1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan
Submit Date2016-04-16
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2016-04-16
Release Version1
Masae Kuboniwa Masae Kuboniwa application/zip

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Collection ID:CO000399
Collection Summary:Cells were collected and washed with Milli-Q water by centrifugation.
Sample Type:Bacterial cells