Summary of Study ST000496

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000373. The data can be accessed directly via it's Project DOI: 10.21228/M8TW22 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000496
Study TitleDistinct signatures of dental plaque metabolic byproducts dictated by periodontal inflammatory status
Study SummaryOnset of chronic periodontitis is associated with an aberrant polymicrobial community, termed dysbiosis. Findings of a recent model of its etiology suggested that dysbiosis holds a conserved metabolic signature as an emergent property. The purpose of this study was to identify robust biomarkers for periodontal inflammation severity. Furthermore, we investigated disease-associated metabolic signatures of periodontal microbiota using a salivary metabolomics approach. Collection of whole saliva samples was performed before and after removal of supragingival plaque (debridement). Periodontal inflamed surface area (PISA) was employed as an indicator of periodontal inflammatory status. Based on multivariate analyses using pre-debridement salivary metabolomics data, we found that the metabolites associated with higher PISA included cadaverine and hydrocinnamate, while uric acid and ethanolamine were associated with lower PISA. Next, we focused on dental plaque metabolic byproducts by selecting significantly decreased salivary metabolites following debridement. Metabolite set enrichment analysis revealed that polyamine metabolism, arginine and proline metabolism, butyric acid metabolism, and lysine degradation were distinctive metabolic signatures of dental plaque in the high PISA group, which may have relevance to the metabolic signatures of disease-associated communities. Collectively, our findings identified potential biomarkers of periodontal inflammatory status, while they also provide insight into metabolic signatures of dysbiotic communities.
Osaka University
DepartmentGraduate School of Dentistry
Last NameKuboniwa
First NameMasae
Address1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan
Submit Date2016-10-23
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2017-11-20
Release Version1
Masae Kuboniwa Masae Kuboniwa application/zip

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Collection ID:CO000511
Collection Summary:The subjects were asked to refrain from brushing and using mouthwash for at least 1 hour prior to sample collection and periodontal examinations. We collected a minimum of 3 mL of unstimulated whole saliva between 1:00 and 3:00 p.m. Thereafter, saliva was sampled again at 15 minutes following removal of supragingival plaque and calculus with an ultrasonic scaler (debridement). Finally, 1 ml of each sample was immediately frozen with liquid nitrogen and stored at -80°C until analysis.
Sample Type:Saliva