Summary of Study ST000569

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000417. The data can be accessed directly via it's Project DOI: 10.21228/M8531V This work is supported by NIH grant, U2C- DK119886.

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Study IDST000569
Study TitleEffect of minimal and complex media on the metabolite profiles
Study SummaryMedia dependent intracellular metabolite changes of microorganisms
Institute
Graduate school of Korea University
Last NameKim
First NameJungyeon
Address145, Anam-ro, Seongbuk-gu, Seoul, Seoul, 02841, Korea, South
Emailkim131812@korea.ac.kr
Phone821082248015
Submit Date2017-03-07
Study CommentsEach class means,Intracellular metabolites of E.coli cultivated in M9 Intracellular metabolites of E.coli cultivated in LB Intracellular metabolites of S.cerevisiae cultivated in YNB Intracellular metabolites of S.cerevisiae cultivated in YPD.
Raw Data AvailableYes
Raw Data File Type(s)peg
Analysis Type DetailGC-MS
Release Date2017-07-10
Release Version1
Jungyeon Kim Jungyeon Kim
https://dx.doi.org/10.21228/M8531V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO000585
Collection Summary:Fast filtration: cells for each experimental condition were obtained by filtering 1 ml of the cell culture using a nylon membrane filter (0.45-µm pore size, Whatman, Piscataway, NJ) under vacuum. Then, the cell mass was washed with 5 ml of distilled water at room temperature. The cells on the membrane filter were quenched in 10 ml of acetonitrile/water (1:1, v/v) extraction solvent at −20 °C.
Sample Type:Bacterial cells
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