Summary of study ST000591

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000432. The data can be accessed directly via it's Project DOI: 10.21228/M86W3V This work is supported by NIH grant, U2C- DK119886.


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Study IDST000591
Study TitleMetablomic profiling in acc1-5 mutant and wild type arabidiopsis
Study SummaryThis experiment tests the metabolic consequence of a mutation at the ACC1 gene (At1g36160). The allele of acc1-5 bearing an EMS mutation, which cause a single amino acid substitution from aspartic acid to asparagine. Seedlings from both the acc1-5 mutant and the wild type were harvested and analyzed via HILIC LC-MS. Of particular interest are metabolites which would be affected by depletion of malonyl-CoA pools (flavenoids) and primary metabolites.
Agriculture and Agri-Food Canada
DepartmentLondon Research and Development Centre
Last NameRenaud
First NameJustin
Address1391 Sandford street, London, Ontario, Canada
Submit Date2017-03-12
PublicationsChen, Chen, et al. "Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis." Nature Plants (2017): 1.
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2017-10-25
Release Version1
Justin Renaud Justin Renaud application/zip

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Collection ID:CO000608
Collection Summary:Arabidopsis seeds obtained from the Arabidopsis Biological Resource Center (ABRC) at the Ohio State University were grown in half strength of Murashige and Skoog (½ MS) medium (0.5XMS salts, 1.5% [w/v] sucrose, and 0.8% agar [pH 5.8]) or soil under 16h/8h light/dark cycle at 23°C. Plants were harvested 12 days after germination. 50 mg of each plant (7 mutants, 7 wild type) were ground in liquid N2 using ball mill and immediately extracted following collection protocol.
Collection Protocol Filename:collection_protocol.pdf
Sample Type:Seedlings
Collection Location:London, Ontario
Collection Frequency:Single