Summary of Study ST000924

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000640. The data can be accessed directly via it's Project DOI: 10.21228/M8Z40Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000924
Study TitleMuRF1-Related Metabolic Alterations in HL-1 Cardiomyocyte Induced by Cyclic Stretch
Study TypeCardiomyocyte cell culture
Study SummaryWe collected cell media and performed GC-MS non-targeted metabolomics to identify the role of MuRF1 in the dynamic metabolic changes in cardiomyocytes.
Institute
University of North Carolina at Chapel Hill
DepartmentPathology & Laboratory Medicine
LaboratoryWillis
Last NameWillis
First NameMonte
Address111 Mason Farm Road
Emailmonte_willis@med.unc.edu
Phone9849995431
Submit Date2017-10-30
Num Groups6
Total Subjects32
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2018-12-11
Release Version1
Monte Willis Monte Willis
https://dx.doi.org/10.21228/M8Z40Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO000956
Collection Summary:Media harvested at 14, 30, and 60 min post stretch (1Hz, 15%)
Collection Protocol ID:None
Collection Protocol Comments:Non-targeted metabolomics analysis. After HL-1 cells were cultured for 16 h in specialized 6well BioFlex culture plates in Claycomb medium, medium was changed to DMEM supplemented 1% penicillin-streptomycin and 10% serum. Transient knockdown of MuRF1 was carried out using recombinant Ad.shRNA MuRF1 at MOI 30. After transduction for 48 h, HL-1 cells were used for mechanical stretch at 15% strain and for different times (15, 30, and 60 min) in a computer-regulated Flexcell FX-5000 Compression System (Flexcell International Corporation, Hillsborough, NC). Non-stretch cells (0 min) were used as the control. After HL-1 cells were stretched, HL-1 cells (at 0 and 60 min) and DMEM medium (at 0, 15, 30, and 60 min) were collected for GC/MS measurement of metabolites, and samples were placed on dry ice/stored at -80C. Samples were then analyzed by GC/MS, as previously described [24]. The raw, transformed, and sorted data used for each of the three comparisons in the metabolomics analyses can be found in Supplemental Table 1. Up to 1 missing value per group was imputed using lowest value in the same group, with groups missing 2 or more excluded from the analysis. The data obtained in this study will be accessible at the NIH Common Fund’s Data Repository and Coordinating Center (supported by NIH grant, U01-DK097430) website, http://www.metabolomicsworkbench.org.
Sample Type:Media
Collection Method:Pipette
Collection Location:Tissue culture.
Collection Frequency:q 15-30 minutes
Collection Duration:1 hour
Volumeoramount Collected:10 ul
Storage Conditions:-80C
Collection Vials:Cryovials
Storage Vials:Cryovials
Collection Tube Temp:Ice
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