Summary of Study ST001149
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000768. The data can be accessed directly via it's Project DOI: 10.21228/M8DH49 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001149 |
| Study Title | Plasmodium Niemann-Pick Type C1-Related Protein is a Druggable Target Required for Parasite Membrane Homeostasis |
| Study Type | Drug Exposure |
| Study Summary | Metabolic examination/confirmation of resistance mutations and GFP tagging observed in study |
| Institute | Pennsylvania State University |
| Department | BMB |
| Laboratory | Llinás Laboratory |
| Last Name | Llinás |
| First Name | Manuel |
| Address | W-122 Millennium Science Complex, Pollock Road |
| eso5005@psu.edu | |
| Phone | 8148673527 |
| Submit Date | 2019-02-15 |
| Raw Data Available | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-05-15 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001208 |
| Collection Summary: | Aspiration of 4 mL media, transfer of sample (1 mL) to 1.5 mL tubes, centrifugation, removal of supernatant, PBS wash, centrifugation, and extraction via 90% methanol with labelled aspartate. |
| Collection Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
| Sample Type: | Cultured cells |
| Collection Method: | Aspiration of 4 mL media, transfer of sample (1 mL) to 1.5 mL tubes |
| Collection Location: | Biological safety hood |
| Collection Frequency: | once after 2.5 hours treatment |
| Collection Duration: | Collection and extraction occurs in under 20 minutes per plate |
| Volumeoramount Collected: | 1 mL containing 1x10^8 infected cells |
| Storage Conditions: | On ice |
| Collection Vials: | 1.5 mL eppendorf |
| Storage Vials: | 1.5 mL eppendorf |
