Summary of study ST001174

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000786. The data can be accessed directly via it's Project DOI: 10.21228/M83398 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001174
Study TitleRole of ClpCP in respiratory and fermentative growth
Study SummaryTo determine metabolite concentrations and differences at the 48 hour time point for WT, ClpC mutant, srrAB mutant, and ClpC:srrAB double mutant
Institute
Montana State University
DepartmentChemistry and Biochemistry
LaboratoryCopie Lab
Last NameEilers
First NameBrian
Address103 Chemistry and Biochemistry Bldg, RM 144, Valerie Copie Lab, Bozeman, Montana, 59717, USA
Emailbrian.eilers@montana.edu
Phone4069945116
Submit Date2019-04-24
Num Groups4 (WT, ClpC, srrAB, and ClpC:srrAB)
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)pdata, .par, .temp, .fid,.scon2, etc
Analysis Type DetailNMR
Release Date2019-05-15
Release Version1
Brian Eilers Brian Eilers
https://dx.doi.org/10.21228/M83398
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001234
Collection Summary:Aerobic growth for all strains was performed on four biological replicates for each cell group. Overnight cultures diluted 1:1000 were used to inoculate 25 mL of fresh TSB in a 250 mL flask with 220 rpm agitation at 37° C. Aliquots of 10 mL were collected at 48 hrs , centrifuged at 5000 rpm for 5 minutes, then rinsed once with 1 mL of 1X PBS, and centrifuged at 5000 rpm for 5 minutes. The supernatant was discarded, and cell pellets were frozen at -80 °C until further use.
Sample Type:Bacterial cells
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