Summary of Study ST001178

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000790. The data can be accessed directly via it's Project DOI: 10.21228/M8K40J This work is supported by NIH grant, U2C- DK119886.

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Study IDST001178
Study TitleMetabolomic analysis of C2C12 myoblasts induced by the transcriptional factor FOXO1
Study SummaryThe transcriptional factor FOXO1 is considered to play roles in the regulation of energy metabolism in various tissues. To determine the metabolic changes occurring due to the activation of FOXO1, we analyzed the metabolic profile of C2C12 myoblasts expressing FOXO1-estrogen receptor fusion protein using CE-TOFMS. In the FOXO1-activated cells, the metabolite levels during glycolysis were higher. In addition, the gene expression of pyruvate dehydrogenase kinase, an enzyme that inhibits glucose utilization, increased. In the FOXO1-activated cells, the metabolite levels of numerous amino acids decreased, with increased gene expression of branched chain amino acid metabolism enzymes. Our results suggest that FOXO1 suppresses glucose utilization and promotes the use of proteins/amino acids as energy sources in muscle cells, potentially during starvation.
Institute
Kyoto Prefectural University
Last NameKamei
First NameYasutomi
Address1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan
Emailcnqwb974@yahoo.co.jp
Phone+81-75-703-5661
Submit Date2019-04-22
Analysis Type DetailCE-MS
Release Date2020-03-03
Release Version1
Yasutomi Kamei Yasutomi Kamei
https://dx.doi.org/10.21228/M8K40J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001238
Collection Summary:Cells were washed with cold PBS and then flash-frozen in liquid N2.
Sample Type:Muscle
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